Anti-Jo-1 antibody levels correlate with disease activity in idiopathic inflammatory myopathy
Anti-Jo-1 antibody levels correlate with disease activity in idiopathic inflammatory myopathy
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DOI：
10.1002/art.22865
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发表时间：
2007-09-01
影响因子：
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通讯作者：
Ascherman, Dana P.
中科院分区：
文献类型：
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作者：
Stone, Kerry B.;Oddis, Chester V.;Ascherman, Dana P.
Objective.以前的病例系列研究了抗jo-1抗体水平和肌炎疾病活动之间的关系，证明了模棱两可的结果。使用酶联免疫吸附试验（ELISA）和肌炎疾病活动的新措施，目前的研究进行系统地重新检查抗Jo-1抗体水平和肌炎的各种疾病表现之间的关联。使用2种独立的ELISA方法定量血清抗jo-1抗体水平，同时使用肌炎疾病活动性评估工具回顾性分级疾病活动性，该工具通过肌炎疾病活动性评估视觉模拟量表（VAS）和肌炎意向治疗指数（MITAX）组件测量7个不同器官系统中的疾病活动性。分别采用斯皮尔曼等级相关系数和混合线性回归分析确定抗jo-1抗体水平与器官特异性疾病活动性之间的关系。对81例抗Jo-1抗体患者的横断面评估显示，抗Jo-1抗体水平与血清肌酸激酶（CK）水平以及肌肉和关节疾病活动性之间存在适度相关性。CK水平（r（s）= 0.38，P = 0.002）、肌炎VAS（r（s）= 0.36，P = 0.002）和关节炎VAS（rs = 0.40，P = 0.001）的相关系数相似。在对11例患者系列样本的多元回归分析中，抗jo-1抗体水平与CK水平显著相关（R-2 = 0.65，P = 0.0002），肌炎VAS（R-2 = 0.53，P = 0.0008），关节炎VAS（R2 = 0.53，P = 0.006），肺部VAS = 0.69，P 0.005），整体VAS（R-2 = 0.63，P = 0.002）和整体MITAX（R-2 = 0.64，P 0.0003）。在这个大系列的特发性炎性肌病患者中，抗jo-1抗体水平与肌肉和关节疾病适度相关，使用重组人Jo-1的定制ELISA证实了这种关联。在一个较小的纵向患者亚组中出现了更显著的相关性，这些患者将抗jo-1抗体水平与肌肉、关节、肺和全身疾病活动联系起来。
Objective. Previous case series have examined the relationship between anti-jo-1 antibody levels and myositis disease activity, demonstrating equivocal results. Using enzyme-linked immunosorbent assays (ELISAs) and novel measures of myositis disease activity, the current study was undertaken to systematically reexamine the association between anti-Jo-1 antibody levels and various disease manifestations of myositis.Methods. Serum anti-jo-1 antibody levels were quantified using 2 independent ELISA methods, while disease activity was retrospectively graded using the Myositis Disease Activity Assessment Tool, which measures disease activity in 7 different organ systems via the Myositis Disease Activity Assessment Visual Analog Scale (VAS) and the Myositis Intention-to-Treat Index (MITAX) components. Spearman's rank correlation coefficients and mixed linear regression analysis were used to identify associations between anti-jo-1 antibody levels and organ-specific disease activity in crosssectional and longitudinal analyses, respectively.Results. Cross-sectional assessment of 81 patients with anti-Jo-1 antibody revealed a modest correlation between the anti-jo-1 antibody level and the serum creatine kinase (CK) level, as well as muscle and joint disease activity. Correlation coefficients were similar for CK levels (r(s) = 0.38, P = 0.002), myositis VAS (r(s) = 0.36, P = 0.002), and arthritis VAS (rs = 0.40, P = 0.001). In multiple regression analyses of 11 patients with serial samples, anti-jo-1 antibody levels correlated significantly with CK levels (R-2 = 0.65, P = 0.0002), myositis VAS (R-2 = 0.53, P = 0.0008), arthritis VAS (R2 = 0.53, P = 0.006), pulmonary VAS, = 0.69, P 0.005), global VAS (R-2 0.63, P = 0.002), and global MITAX (R-2 = 0.64, P 0.0003).Conclusion. In this large series of patients with idiopathic inflammatory myopathy, anti-jo-1 antibody levels correlated modestly with muscle and joint disease, an association confirmed by a custom ELISA using recombinant human Jo-1. More striking associations emerged in a smaller longitudinal subset of patients that link anti-jo-1 antibody levels to muscle, joint, lung, and global disease activity.