Aptamer-conjugated nanoparticles for selective collection and detection of cancer cells
Aptamer-conjugated nanoparticles for selective collection and detection of cancer cells
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DOI：
10.1021/ac052015r
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发表时间：
2006-05-01
影响因子：
7.4
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
Herr, Joshua K.;Smith, Joshua E.;Tan, Weihong
我们已经开发了一种快速收集和检测白血病细胞的方法，使用一种新型的两纳米颗粒检测方法，以适配体作为分子识别元件。我们的实验室使用基于细胞的SELEX策略选择了CCRF-CEM急性白血病细胞的适配体序列，当应用该方法时，可以从复杂的混合物（包括全血样本）中特异性识别细胞。采用核酸适配体修饰的磁性纳米粒子进行靶细胞提取，同时加入核酸适配体修饰的荧光纳米粒子进行敏感细胞检测。结合两种类型的；纳米粒子允许快速，选择性和灵敏的检测，这是单独使用任何一种粒子所不可能的。荧光纳米粒子放大了对应于单个适体结合事件的信号强度，从而提高了使用单个染料标记探针的方法的灵敏度。此外，适配体修饰的磁性纳米颗粒允许快速提取目标细胞，这是其他分离方法无法实现的。荧光成像和流式细胞术用于细胞检测，以证明该方法在医学诊断中的潜在应用。
We have developed a method for the rapid collection and detection of leukemia cells using a novel two-nanoparticle assay with aptamers as the molecular recognition element An aptamer sequence was selected using a cell-based SELEX strategy in our laboratory for CCRF-CEM acute leukemia cells that, when applied in this method, allows for specific recognition of the cells from complex mixtures including whole blood samples. Aptamer-modified magnetic nanoparticles were used for target cell extraction, while aptamer-modified fluorescent nanoparticles were simultaneously added for sensitive cell detection. Combining two types; of nanoparticles allows for rapid, selective, and sensitive detection not possible by using either particle alone. Fluorescent nanoparticles amplify the signal intensity corresponding to a single aptamer binding event, resulting in improved sensitivity over methods using individual dye-labeled probes. In addition, aptamer-modified magnetic nanoparticles allow for rapid extraction of target cells not possible with other separation methods. Fluorescent imaging and flow cytometry were used for cellular detection to demonstrate the potential application of this method for medical diagnostics.