Genome-wide Characterization and Expression Analysis of Sugar Transporter Family Genes in Woodland Strawberry
Genome-wide Characterization and Expression Analysis of Sugar Transporter Family Genes in Woodland Strawberry
复制标题
林地草莓糖转运蛋白家族基因的全基因组表征和表达分析
DOI：
10.3835/plantgenome2017.11.0103
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发表时间：
2018-11-01
期刊：
影响因子：
4.2
通讯作者：
Fang, Jinggui
中科院分区：
文献类型：
--
作者：
Jiu, Songtao;Haider, Muhammad Salman;Fang, Jinggui
在高等植物中，糖是营养物质和重要的信号分子。糖转运蛋白（ST）促进糖跨膜转运，并与装载和卸载的导电复合物。草莓（Fragaria xananassa Duchesne ex Rozier）是世界上重要的经济作物之一，也是世界上广泛栽培的肉质水果模式植物。本研究以66个林地草莓（F. vesca L.）通过系统发育分析，进一步将森林草莓基因组中的ST（FvST）基因分为8个不同的亚家族。在FvST基因家族的启动子序列中，对顺式调节元件的搜索表明，其中一些可能受植物激素调节（例如，水杨酸，脱落酸，和生长素），非生物的（例如，干旱、过冷和光照）和生物胁迫因素。外显子-内含子分析表明，每个亚家族表现出密切相关的基因结构特征的基础上相似的数量或长度的外显子。此外，为了理解FvST基因家族的潜在进化机制，还对基因组重复事件进行了分析。片段和串联重复分析表明，一些ST基因是通过全基因组重复（WGD）或片段重复伴随串联重复而产生的。对24个FvST基因在营养生长期和果实发育期的表达分析表明，几个ST基因的表达具有组织和发育阶段特异性。总的来说，我们的研究结果是重要的，在理解从源到库细胞的光同化物的分配，并提供洞察林地草莓FvST基因家族的基因组组织和表达谱。
In higher plants, sugars are nutrients and important signal molecules. Sugar transporters (STs) facilitate sugar transport across membranes and are associated with loading and unloading of the conducting complex. Strawberry (Fragaria xananassa Duchesne ex Rozier) is one of the most economically important and widely cultivated fruit crop and a model plant among fleshy fruits worldwide. In this study, 66 woodland strawberry (F. vesca L.) ST (FvST) genes were identified and further classified into eight distinct subfamilies in the woodland strawberry genome based on the phylogenetic analysis. In the promoter sequences of FvST gene families, a search for cis-regulatory elements suggested that some of them might probably be regulated by plant hormones (e.g., salicylic acid, abscisic acid, and auxin), abiotic (e.g., drought, excessive cold, and light), and biotic stress factors. Exon-intron analysis showed that each subfamily manifested closely associated gene architectural features based on similar number or length of exons. Moreover, to comprehend the potential evolution mechanism of FvST gene family, the analysis of genome duplication events was performed. The segmental and tandem duplication analysis elucidated that some of ST genes arose through whole-genome duplication (WGD) or segmental duplication, accompanied by tandem duplications. The expression analysis of 24 FvST genes in vegetative and during fruit development has shown that the expression of several ST genes was tissue and developmental stage specific. Generally, our findings are important in understanding of the allocation of photo assimilates from source to sink cell and provide insights into the genomic organization and expression profiling of FvST gene families in woodland strawberry.