Gene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction.
Gene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction.
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OmpA 蛋白的基因结构，OmpA 蛋白是细胞与细胞相互作用所需的大肠杆菌的主要表面蛋白。
DOI：
10.1016/0022-2836(80)90193-x
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发表时间：
1980
影响因子：
5.6
通讯作者：
Inouye,M
中科院分区：
文献类型：
--
作者：
Movva,NR;Nakamura,K;Inouye,M
测定了大肠杆菌的主要外膜蛋白OmpA基因的DNA序列。推测该蛋白的信使RNA由5‘端非翻译区140个碱基、编码区1038个碱基和3’端非翻译区46个碱基组成。在该基因中，除了核糖体结合位点和起始密码子周围的99个碱基外，可以形成25个稳定的茎和环结构(总ΔG=−215kcal)，覆盖整个基因。该基因3‘端的二级结构在终止密码子和转录终止信号方面与另一种主要的外膜蛋白脂蛋白的mRNA有显著的相似性。从DNA序列中发现，OmpA蛋白由325个氨基酸残基组成，由分泌前体-OmpA蛋白产生，在氨基末端有一个额外的21个氨基酸残基的信号肽。密码子的使用很不寻常，高达76%的氨基酸残基仅由17个密码子编码。还测定了克隆载体pMF21上一个未知基因与TheompA基因融合基因的DNA序列。在融合基因产生的杂交蛋白中，OmpA蛋白羧基末端的96个残基被pMF21基因的5个残基取代。然而，杂合蛋白仍然具有对OmpA蛋白特异性噬菌体的敏感性，这表明OmpA蛋白可以在没有30%的蛋白质从其羧基末端进入外膜的情况下被掺入。
The DNA sequence of the gene for the ompA protein, a major outer membrane protein ofEscherichia coli, has been determined. The messenger RNA for the protein was deduced to consist of 140, 1038 and 46 bases for the 5′ end untranslated, the coding and the 3′ end untranslated regions, respectively. In the mRNA, 25 stable stem-and-loop structures (totalΔG= −215 kcal) can be formed, which cover the entire mRNA except for a sequence of 99 bases around the ribosome-binding site and the initiation codon. The secondary structure at the 3′ end of the mRNA shows a remarkable similarity in respect of the termination codon and the transcription termination signal with the mRNA for the lipoprotein, another major outer membrane protein. From the DNA sequence, the ompA protein was found to consist of 325 amino acid residues and is produced from a secretory precursor, pro-ompA protein, having a signal peptide of 21 extra amino acid residues at the amino terminal end. The codon usage is unusual and as much as 76% of the total amino acid residues are coded only by 17 codons.The DNA sequence of a fused gene between theompAgene and an unknown gene from a cloning vector, pMF21, was also determined. In the hybrid protein produced by the fused gene 96 residues from the carboxyl terminus of the ompA protein were replaced with five residues from the gene from pMF21. However, the hybrid protein can still confer the sensitivity against ompA protein-specific phage, suggesting that the ompA protein can be incorporated without 30% of the protein from its carboxyl terminus into the outer membrane.