Photothermal-Responsive Conjugated Polymer Nanoparticles for Remote Control of Gene Expression in Living Cells
Photothermal-Responsive Conjugated Polymer Nanoparticles for Remote Control of Gene Expression in Living Cells
复制标题
用于远程控制活细胞基因表达的光热响应共轭聚合物纳米颗粒
DOI：
10.1002/adma.201705418
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发表时间：
2018
影响因子：
29.4
通讯作者：
Wang Shu
中科院分区：
文献类型：
--
作者：
Wang Yunxia;Li Shengliang;Zhang Pengbo;Bai Haotian;Feng Liheng;Lv Fengting;Liu Libing;Wang Shu
细胞生物过程的远程控制和非侵入性操作作为一种强有力的细胞功能调控技术受到了广泛关注。在这里，开发了一种策略，通过使用光热响应共轭聚合物纳米颗粒（CPNs）作为近红外光照射下的换能器，以高空间和时间分辨率远程控制细胞内基因表达。经带正电荷的多肽修饰后，具有上级光热转换能力的CPNs能够有效地包裹在活细胞表面，并产生局部热，触发靶基因的表达。热诱导型热休克蛋白-70启动子启动下游EGFP基因的转录以响应热休克，从而在活细胞中产生绿色荧光蛋白。热诱导基因启动子和光热响应CPNs的结合为热遗传学的发展提供了一种方法。
Remote control and noninvasive manipulation of cellular bioprocess has received intensive attention as a powerful technology to control cell functions. Here, a strategy is developed to remotely control intracellular gene expression with high spatial and temporal resolutions by using photothermal‐responsive conjugated polymer nanoparticles (CPNs) as the transducer under near‐infrared light irradiation. After being modified with positive charged peptide, the CPNs with superior photothermal conversion capacity could effectively coat on the surface of living cells and generate localized heat to trigger target gene expression. The heat‐inducible heat shock protein‐70 promoter starts transcription of downstream EGFP gene in response to heat shock, thus producing green fluorescent protein in the living cells. The combination of heat‐inducible gene promoter and photothermal‐responsive CPNs provides a method for the development of thermogenetics.