Butane metabolism by butane-grown 'Pseudomonas butanovora'
Butane metabolism by butane-grown 'Pseudomonas butanovora'
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DOI：
10.1099/13500872-145-5-1173
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发表时间：
1999-05-01
期刊：
影响因子：
2.8
通讯作者：
Arp, DJ
中科院分区：
文献类型：
--
作者：
Arp, DJ
丁烷生长的“噬丁烷假单胞菌”的丁烷代谢途径被确定为丁烷-> 1-丁醇->丁醛->丁酸。丁烷最初在末端碳处氧化以产生1-丁醇。当细胞在5 mM 1-丙醇存在下孵育时，高达90%的消耗的丁烷被认为是1-丁醇（以阻断1-丁醇的后续代谢）。即使在5 mM 2-戊醇（2-丁醇消耗的有效抑制剂）存在下，也未检测到亚末端氧化产物2-丁醇的产生。乙烷、丙烷和戊烷也被氧化，但甲烷没有被氧化。丁烷生长的细胞消耗1-丁醇和其他末端醇。仲醇，包括2-丁醇，被氧化成相应的酮。丁醛被进一步氧化成丁酸盐，如通过用1 mM戊酸钠阻断丁酸盐代谢所证明的。当细胞与1 mM戊酸钠孵育时，丁酸盐也从丁烷中积累。途径中间产物（丁烷、1-丁醇、丁醛和丁酸盐）和2-丁醇刺激丁烷生长的细胞的O-2消耗，1-丁醇、丁醛和丁酸盐支持'P. butanovora'的生长，2-丁醇和乳酸盐也是如此。
The pathway of butane metabolism by butane-grown 'Pseudomonas butanovora' was determined to be butane --> 1-butanol --> butyraldehyde --> butyrate. Butane was initially oxidized at the terminal carbon to produce 1-butanol. Up to 90% of the butane consumed was accounted for as 1-butanol when cells were incubated in the presence of 5 mM 1-propanol (to block subsequent metabolism of 1-butanol). No production of the subterminal oxidation product, 2-butanol, was detected, even in the presence of 5 mM 2-pentanol (an effective inhibitor of 2-butanol consumption). Ethane, propane and pentane, but not methane, were also oxidized. Butane-grown cells consumed 1-butanol and other terminal alcohols. Secondary alcohols, including 2-butanol, were oxidized to the corresponding ketones. Butyraldehyde was further oxidized to butyrate as demonstrated by blocking butyrate metabolism with 1 mM sodium valerate. Butyrate also accumulated from butane when cells were incubated with 1 mM sodium valerate. The pathway intermediates (butane, 1-butanol, butyraldehyde and butyrate) and 2-butanol stimulated O-2 consumption by butane-grown cells, 1-Butanol, butyraldehyde and butyrate supported growth of 'P. butanovora', as did 2-butanol and lactate.