Comparison between spoligotyping and IS6110 restriction fragment length polymorphisms in molecular genotyping analysis of Mycobacterium tuberculosis strains
Comparison between spoligotyping and IS6110 restriction fragment length polymorphisms in molecular genotyping analysis of Mycobacterium tuberculosis strains
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DOI：
10.1016/j.mcp.2005.01.001
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发表时间：
2005-08-01
影响因子：
3.3
通讯作者：
Franzetti, F
中科院分区：
文献类型：
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作者：
Gori, A;Degli Esposti, A;Franzetti, F
在结核分枝杆菌菌株的鉴定中，将 Spoligotyping 与 RFLP 指纹分析进行了比较。 Spoligotyping.. 敏感性为 97.6%，特异性为 47%。通过 spoligotyping 聚类的两个菌株也通过 RFLP 分析聚类的总体概率为 33%；通过 RFLP 分析聚类的两个菌株也通过 spoligotyping 分析聚类的概率为 95%。然而，比较五次爆发事件中的两种方法，证明 spoligotyping 和 RFLP 之间完全一致。此外，我们评估了来自加勒比国家、伦敦和古巴的 spoligotyping 数据库中 17 个最大的 spoligotyping 集群的存在情况。所有队列中仅存在 17 种模式中的 5 种。比较与这些模式相关的 spoligotyping 和 RFLP 方法的条件概率导致非常低的一致性（范围从 2% 到 38%）。总之，我们确认单独使用 spoligotyping 会高估近期传播的数量，并且不代表适合广泛临床实践应用的方法。然而，它可以在新的环境中以及在更局部或受限的环境中获得菌株身份的初步良好了解，与 RFLP 相比，达到相同结果的概率为 100%，证实了 spoligotyping 在流行病事件管理中的有用性，特别是在医院、监狱和封闭社区中。 (C) 2005 Elsevier Ltd. 保留所有权利。
Spoligotyping was compared with RFLP fingerprinting analysis in the identification of Mycobacterium tuberculosis strains. Spoligotyping.. sensitivity was 97.6% with a specificity of 47%. The global probability for two strains clustered with spoligotyping to be clustered also with RFLP analysis was 33%; the probability for two strains clustered with RFLP analysis to be clustered also with spoligotyping analysis was 95%. However, comparing the two methods in five outbreak episodes, full concordance was evidenced between spoligotyping and RFLP. Moreover, we evaluated the presence of our 17 largest spoligotyping clusters in spoligotyping databases from Caribbean countries, London and Cuba. Only five out of 17 patterns were present in all the cohorts. The conditional probability comparing spoligotyping and RFLP methods related to these patterns resulted in very low concordance (range from 2 to 38%). In conclusion, we confirm that spoligotyping when used alone overestimates the number of recent transmission and does not represent a suitable method for wide clinical practice application. However, it allows to get a first good picture of strain identity in a new setting and in more localized or confined settings, the probability of reaching the same result compared to RFLP was 100% confirming the usefulness of spoligotyping in the management of epidemic events, especially in hospitals, prisons and close communities. (C) 2005 Elsevier Ltd. All rights reserved.