The changes in monosaccharide composition of immunoglobulin G in the course of rheumatoid arthritis
The changes in monosaccharide composition of immunoglobulin G in the course of rheumatoid arthritis
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DOI：
10.1007/s10067-006-0370-7
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发表时间：
2007-05-01
影响因子：
3.4
通讯作者：
Sierakowski, Stanislaw
中科院分区：
文献类型：
--
作者：
Gindzienska-Sieskiewicz, Ewa;Klimiuk, Piotr Adrian;Sierakowski, Stanislaw
本研究的目的是确定类风湿关节炎(RA)患者免疫球蛋白G(Ig G)的半乳糖化是否与病情严重程度和病程有关。对50例类风湿关节炎(RA)患者和30例健康体检者的血清Ig G糖基化水平进行了分析。从血清中提取免疫球蛋白，用气相色谱法测定其单糖组成。计算半乳糖/甘露糖含量的比值。根据Steinbrocker的标准，患者根据三个不同的标准进行分组：病程、RA严重程度(疾病活动评分指数)和放射学疾病进展程度。在类风湿关节炎患者中，与健康对照组相比，半乳糖比率显著降低(P<0.01)。在RA病程长(超过15年)的患者中，观察到与关节炎患者相比，半乳糖(p<；0.05)比率显著降低。对于重症类风湿性关节炎患者组，我们发现与缓解组患者相比，半乳糖(p<；0.001)比率降低。根据Steinbrocker的说法，对于放射学IV期的患者，每条寡糖链的Ig G半乳糖(p<；0.01)含量也比那些I期RA患者下降得更多。类风湿关节炎患者血清中半乳糖化水平和免疫球蛋白水平均明显降低。免疫球蛋白这种碳水化合物成分的缺乏与类风湿性关节炎的严重程度和病程相关，可用于监测早期关节炎的进展。
The objective of this study was to determine whether galactosylation of immunoglobulin G (IgG) in patients with rheumatoid arthritis (RA) correlates with severity and duration of illness. Serum IgG glycosylation from 50 patients with RA in comparison with 30 healthy controls was analyzed. IgG from sera was isolated and monosaccharide composition was determined by means of gas chromatography. Ratio of galactose to mannose content was calculated. Patients were divided into groups according to three different criteria: disease duration, severity of RA (disease activity score index), and radiological degree of advancement of illness according to Steinbrocker. In patients with RA, significant decrease (p < 0,01) of galactose ratio was observed in comparison with healthy control. In patients with long duration of RA (more than 15 years), significant decrease of galactose (p < 0.05) ratio in comparison with patients who have had arthritis for less then 5 years was observed. For the group of patients with severe RA, we found reduction of galactose (p < 0.001) ratio vs the group of patients in remission. For those patients who had radiological stage IV according to Steinbrocker, IgG galactose (p < 0.01) content per oligosaccharide chain were also more decreased than in those patients who had stage I RA. Decreased galactosylation and of IgG in RA was observed. The lack of this carbohydrate component of IgG correlates with severity and duration of RA and could be used in monitoring the progression in early arthritis.