Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication.
Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication.
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DOI：
10.1016/j.molcel.2018.08.010
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发表时间：
2018-10-18
期刊：
影响因子：
16
通讯作者：
Groth A
中科院分区：
文献类型：
--
作者：
Reverón-Gómez N;González-Aguilera C;Stewart-Morgan KR;Petryk N;Flury V;Graziano S;Johansen JV;Jakobsen JS;Alabert C;Groth A
在DNA复制过程中，染色质组织在全基因组范围内被破坏。在新合成的DNA上，核小体由新的天然组蛋白和旧的修饰组蛋白组装而成。目前还不清楚组蛋白翻译后修饰（PTM）的景观是否在DNA复制过程中忠实地复制或表观基因组受到干扰。在这里，我们开发了复制后染色质占用率（ChOR-seq），以确定DNA复制后立即和整个细胞周期的组蛋白PTM占用率。我们发现H3 K4 me 3，H3 K36 me 3，H3 K79 me 3和H3 K27 me 3的位置信息通过组蛋白回收在新合成的DNA上以高精度再现。定量ChOR-seq揭示了恢复H3 K4 me 3和H3 K27 me 3水平的从头甲基化在整个细胞周期中发生，具有标记和基因座特异性动力学。总的来说，这表明，准确的亲本组蛋白再循环保留了位置信息，并允许PTM传递到子细胞，而新组蛋白的修饰引起了整个细胞周期中复杂的表观基因组波动，这可能是细胞间异质性的基础。ChOR-seq确定组蛋白PTM在新复制的DNA上的占有率组蛋白PTM位置信息通过亲本组蛋白再循环而被保留亲本H3 K27 me 3结构域是稳定的并且遗传给子细胞组蛋白PTM水平的恢复遵循标记和基因座特异性动力学组蛋白修饰是表观基因组的核心组分。Reverón-Gómez等人开发ChOR-seq来分析DNA复制后的组蛋白修饰，并发现修饰的亲本组蛋白的基因组定位保留在子链上，而新的组蛋白修饰以恢复复制前水平遵循标记和基因座特异性动力学。
Chromatin organization is disrupted genome-wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naive histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is faithfully copied during DNA replication or the epigenome is perturbed. Here we develop chromatin occupancy after replication (ChOR-seq) to determine histone PTM occupancy immediately after DNA replication and across the cell cycle. We show that H3K4me3, H3K36me3, H3K79me3, and H3K27me3 positional information is reproduced with high accuracy on newly synthesized DNA through histone recycling. Quantitative ChOR-seq reveals that de novo methylation to restore H3K4me3 and H3K27me3 levels occurs across the cell cycle with mark- and locus-specific kinetics. Collectively, this demonstrates that accurate parental histone recycling preserves positional information and allows PTM transmission to daughter cells while modification of new histones gives rise to complex epigenome fluctuations across the cell cycle that could underlie cell-to-cell heterogeneity. ChOR-seq determines histone PTM occupancy on newly replicated DNA Histone PTM positional information is preserved through parental histone recycling Parental H3K27me3 domains are stable and inherited to daughter cells Restoration of histone PTM levels follows mark- and locus-specific kinetics Histone modifications are a core component of the epigenome. Reverón-Gómez et al. develop ChOR-seq to profile histone modifications after DNA replication and find that the genomic localization of modified parental histones is preserved on daughter strands while new histone modification to restore pre-replication levels follows mark- and locus-specific kinetics.
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影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
影响因子：
16.8
作者：
Højfeldt JW;Laugesen A;Willumsen BM;Damhofer H;Hedehus L;Tvardovskiy A;Mohammad F;Jensen ON;Helin K
通讯作者：
Helin K
影响因子：
14.9
作者：
Fennessy, Ross T.;Owen-Hughes, Tom
通讯作者：
Owen-Hughes, Tom
影响因子：
10.5
作者：
Alabert C;Barth TK;Reverón-Gómez N;Sidoli S;Schmidt A;Jensen ON;Imhof A;Groth A
通讯作者：
Groth A
影响因子：
2.9
作者：
JACKSON, V
通讯作者：
JACKSON, V