Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control.
Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control.
复制标题
直接电刺激生肌培养物，用于分析肌纤维类型控制。
DOI：
10.1007/978-1-61779-343-1_5
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
DiMario,JosephX
中科院分区：
文献类型：
--
作者：
Cavanaugh,EricJ;Crew,JenniferR;DiMario,JosephX
Secondary skeletal muscle fiber phenotype is dependent upon depolarization from motor neuron innervation. To study the effects of depolarization on muscle fiber type development, several in vivo and in vitro model systems exist. We have developed a relatively simple-to-use in vitro model system in which differentiated muscle cells are directly electrically stimulated at precise frequencies. This allows for single cell analysis as well as biochemical and molecular analyses of the mechanisms that control skeletal muscle phenotype.
