Physico-chemical oxidative cleavage strategy facilitates the degradation of recalcitrant crystalline cellulose by cellulases hydrolysis.
Physico-chemical oxidative cleavage strategy facilitates the degradation of recalcitrant crystalline cellulose by cellulases hydrolysis.
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物理化学氧化裂解策略促进纤维素酶水解降解顽固的结晶纤维素
DOI：
10.1186/s13068-018-1016-0
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发表时间：
2018
影响因子：
6.3
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Zhou H;Zhou H;Wang L;Liu Y
柠檬酸结晶纤维素的有效酶促转化对于实现纤维素生物质到生物燃料和化学品的成本有效的工业转化是至关重要的。充分认识酶消化机理，为设计高效的生物质转化工艺提供了新的途径。因此，一个持续的驱动器是鼓舞人心的，以发现新的路线，以促进结晶纤维素disruption. ResultsIn本文中，物理化学氧化裂解策略的辐射氧化/后还原（IOPR）被用来处理结晶纤维素I切割糖苷键协会与一些新的氧化和还原链端，从而提高下游降解纤维素酶从里氏木霉。研究了不同温度、不同温度下处理后结晶纤维素的水解性能。reeseiCel 7A（TrCel 7A）或纤维素酶混合物（90%Celluclast 1.5L，10%0-葡糖苷酶）。81.6和/或97%的转化效率已分别达到24小时和48小时的纤维素酶水解。结晶纤维素在IOPR后的高效转化主要归因于产生了一些新的链端，这些新的链端通过MAIDI-TOF-MS和HPLC进行了鉴定。此外，还利用XRD、EPR、ATR-FTIR、GPC和XPS等技术对结晶纤维素在IOPR前后的纳米级结构进行了系统的研究。与TEM图像一起，结果揭示了一个迷人的消化机制的“剥离”和“腔形成”的范例对降解结晶纤维素的纤维素酶混合物IOPR treatment.ConclusionsThis令人鼓舞的结果表明，建议IOPR方法将成为一个潜在的竞争力替代目前的生物质预处理。这为木质纤维素生物炼制中预处理的实施和酶混合物的设计开辟了新的途径。
BackgroundEfficient enzymatic conversion of recalcitrant crystalline cellulose is critical for enabling cost-effective industrial conversion of cellulosic biomass to biofuels and chemicals. Fully understanding enzyme digestion mechanism is paving a new way to design efficient process for biomass conversion. Accordingly, a continuing drive is inspiring to discover new routes to promote crystalline cellulose disruption.ResultsHerein, a physico-chemical oxidative cleavage strategy of irradiation oxidation/post-reduction (IOPR) was employed to treat crystalline cellulose I to cleave glycosidic bonds association with some new oxidized and reduced chain ends, thus boosting downstream degradation by cellulases fromTrichoderma reesei. The hydrolysis performance of treated crystalline cellulose was conducted with eitherT. reeseiCel7A (TrCel7A) alone, or a cellulase enzyme mixture (90% Celluclast 1.5 L, 10% β-glucosidase). 81.6 and/or 97% of conversion efficiency have been reached for 24-h and 48-h cellulase hydrolysis, respectively. The high efficient conversion of crystalline cellulose after IOPR is mainly attributed to generating some new chain ends, which are identified by MAIDI-TOF–MS and HPLC. Furthermore, the nanoscale architectures of crystalline cellulose before and after IOPR are systematically investigated by XRD, EPR, ATR- FTIR, GPC, and XPS techniques. Together with TEM images, the results reveal a fascinating digestion mechanism of “peel-off” and “cavity-formation” paradigms toward degrading crystalline cellulose by cellulase mixtures after IOPR treatment.ConclusionsThis encouraging results show that the proposed IOPR approach will become a potential competitive alternative to current biomass pretreatment. It opens a new avenue toward the implementation of pretreatment and the design of enzyme cocktails in lignocellulosic biorefinery.
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作者：
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