Complete Sequence Analysis of Two Methanotroph-Specific repABC-Containing Plasmids from Methylocystis sp Strain SC2
Complete Sequence Analysis of Two Methanotroph-Specific repABC-Containing Plasmids from Methylocystis sp Strain SC2
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DOI：
10.1128/aem.00628-12
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发表时间：
2012-06-01
影响因子：
4.4
通讯作者：
Liesack, Werner
中科院分区：
文献类型：
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作者：
Dam, Bomba;Kube, Michael;Liesack, Werner
在甲烷氧化细菌甲基孢囊菌属SC2菌株的全基因组鸟枪测序组装期间，测定了229.6 kb（pBSC 2 -1）和143.5 kb（pBSC 2 -2）的两个大的低拷贝数质粒的完整核苷酸序列。通过脉冲场凝胶电泳和Southern杂交证实了SC2菌株中两种质粒的物理存在。两种质粒都具有repABC系统的保守复制模块，并携带参与其忠实维持和接合的基因。此外，它们含有可能参与基本代谢过程的基因。这些基因包括pBSC 2 -1中的几个重金属抗性基因和铜转运基因，以及pBSC 2 -2中的完整的一氧化二氮还原酶操纵子和pmoC单体，后者编码颗粒甲烷单加氧酶的PmoC亚基。
The complete nucleotide sequences of two large, low-copy-number plasmids of 229.6 kb (pBSC2-1) and 143.5 kb (pBSC2-2) were determined during assembly of the whole-genome shotgun sequences of the methane-oxidizing bacterium Methylocystis sp. strain SC2. The physical existence of the two plasmids in strain SC2 was confirmed by pulsed-field gel electrophoresis followed by Southern hybridization. Both plasmids have a conserved replication module of the repABC system and carry genes involved in their faithful maintenance and conjugation. In addition, they contain genes that might be involved in essential metabolic processes. These include several heavy metal resistance genes and copper transport genes in pBSC2-1 and a complete nitrous oxide reductase operon and a pmoC singleton in pBSC2-2, the latter encoding the PmoC subunit of particulate methane monooxygenase.