Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats
Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats
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DOI：
10.1203/pdr.0b013e3182096ff0
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发表时间：
2011-03-01
影响因子：
3.6
通讯作者：
Mack, David R.
中科院分区：
文献类型：
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作者：
Dykstra, Natalie S.;Hyde, Lucie;Mack, David R.
在摄入后，益生菌可以通过许多保护机制来保护宿主，包括调节参与肠先天粘膜防御的基因，例如上皮细胞来源的粘蛋白糖蛋白和凋亡蛋白抑制剂。为了确定体内效应的特异性和反应的持续性，将植物乳杆菌299 v（Lp 299 v）、鼠李糖乳杆菌R 0011（LrR 0011）和两歧双歧杆菌R 0071（BbR 0071）重复或间歇地添加到Sprague-Dawley大鼠的饮用水中。采用RT-PCR和免疫组织化学方法检测Muc 2、Muc 3、神经元凋亡抑制蛋白（NAIP）、人凋亡抑制蛋白1/细胞凋亡抑制蛋白2（HIAP 1/cIAP 2）、人凋亡抑制蛋白2/细胞凋亡抑制蛋白1（HIAP 2/cIAP 1）mRNA和蛋白水平。活Lp 299 v、BbR 0071和LrR 0011增加空肠和回肠中Muc 3蛋白和mRNA的表达。热灭活和非粘附衍生物的Lp 299 v未能诱导Muc 3的表达。Lp 299 v诱导HIAP 2/cIAP 1和NAIP表达。Muc 3粘蛋白的表达升高5天后，口服给药的Lp 299 v，然而，这种效果并没有持续，尽管持续每天摄入的益生菌。然而，间歇性脉冲摄入益生菌会反复增加Muc 3的表达。我们的结论是，选定的益生菌可以诱导粘膜肠上皮细胞的保护基因，脉冲益生菌管理的效果是可重复的。（Pediatr Res 69：206-211，2011）
Upon ingestion, probiotics may act to protect the host through a number of protective mechanisms including modulation of genes involved in intestinal innate mucosal defense such as epithelial cell-derived mucin glycoproteins and inhibitor of apoptosis proteins. To determine the specificity of effect and sustainability of response in vivo, Lactobacillus plantarum 299v (Lp299v), Lactobacillus rhamnosus R0011 (LrR0011), and Bifidobacterium bifidum R0071 (BbR0071) were added repeatedly or intermittently to the drinking water of Sprague-Dawley rats. After killing the rats via CO, suffocation, Muc2, Muc3, neuronal apoptosis inhibitor protein (NAIP), human inhibitor of apoptosis protein 1/cellular inhibitor of apoptosis 2 (HIAP1/cIAP2), and human inhibitor of apoptosis protein 2/cellular inhibitor of apoptosis 1 (HIAP2/cIAP1) mRNA and protein levels were analyzed via RT-PCR and immunohistochemistry. Live Lp299v, BbR0071, and LrR0011 increased Muc3 protein and mRNA expression in jejunum and ileum. Heat-killed and a nonadherent derivative of Lp299v failed to induce Muc3 expression. Lp299v did induce expression of HIAP2/cIAP1 and NAIP expression. Muc3 mucin expression was elevated for 5 d after oral administration of Lp299v; however, this effect was not sustained despite ongoing daily ingestion of a probiotic. Intermittent pulse ingestion of probiotics, however, was found to repeatedly increase Muc3 expression. We conclude that selected probiotics can induce protective genes of mucosal intestinal epithelial cells, an effect that is reproducible with pulse probiotic administration. (Pediatr Res 69: 206-211, 2011)