Youngiasides A and C Isolated from Youngia denticulatum Inhibit UVB-Induced MMP Expression and Promote Type I Procollagen Production via Repression of MAPK/AP-1/NF-κB and Activation of AMPK/Nrf2 in HaCaT Cells and Human Dermal Fibroblasts
Youngiasides A and C Isolated from Youngia denticulatum Inhibit UVB-Induced MMP Expression and Promote Type I Procollagen Production via Repression of MAPK/AP-1/NF-κB and Activation of AMPK/Nrf2 in HaCaT Cells and Human Dermal Fibroblasts
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DOI：
10.1021/acs.jafc.5b00467
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发表时间：
2015-06-10
影响因子：
6.1
通讯作者：
Nho, Chu Won
中科院分区：
文献类型：
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作者：
Kim, Myungsuk;Park, Young Gyun;Nho, Chu Won
本研究探讨了黄芪甲苷（YA）、黄芪甲苷C（YC）和黄姜提取物（YDE）对体外衰老的影响，并在UVB照射的HaCaT角质形成细胞和人皮肤成纤维细胞（HDF）中评估了其分子机制。结果显示，YA、YC和YDE降低HaCaT细胞和HDFs中基质金属蛋白酶（MMP）的表达和产生，并增加HDFs中胶原的表达和产生。此外，YA，YC和YDE显着增加抗氧化酶的表达，从而下调UVB诱导的活性氧（ROS）的生产和ROS诱导的丝裂原活化蛋白激酶（MAPK）和激活蛋白-1（AP-1）信号在HaCaT细胞。此外，YA，YC，和YDE减少I？Ba和IKK α/β阻断核因子-κ B（NF-κ B）p65核转位，并强烈抑制促炎介质。最后，YA，YC，和YDE增强UVB诱导的腺苷一磷酸活化蛋白激酶（AMPK）磷酸化和YA和YC没有抑制MMP-1的生产AMPK抑制剂或核因子红细胞2相关因子-2（Nrf 2）siRNA处理的HaCaT细胞。结果表明，这些化合物可能是预防和治疗皮肤光老化的潜在治疗剂。
This study investigated the effects of youngiaside A (YA), youngiaside C (YC), and Youngia denticulatum extract (YDE) on extrinsic aging and assessed its molecular mechanisms in UVB-irradiated HaCaT keratinocytes and human dermal fibroblasts (HDFs). The results showed that YA, YC, and YDE decreased matrix metalloproteinase (MMP) expression and production in HaCaT cell and HDFs and increased collagen expression and production in HDFs. In addition, YA, YC, and YDE significantly increased antioxidant enzyme expression, thereby down-regulating UVB-induced reactive oxygen species (ROS) production and ROS-induced mitogen-activated protein kinase (MAPK) and activator protein-1 (AP-1) signaling in HaCaT cells. Furthermore, YA, YC, and YDE reduced phosphorylation of I?Ba and IKK alpha/beta, blocked nuclear factor-kappa B (NF-kappa B) p65 nuclear translocation, and strongly suppressed pro-inflammatory mediators. Finally, YA, YC, and YDE augmented UVB-induced adenosine monophosphate activated protein kinase (AMPK) phosphorylation and YA and YC did not inhibit MMP-1 production in AMPK inhibitor or nuclear factor-erythroid 2-related factor-2 (Nrf2) siRNA-treated HaCaT cells. The results suggest that these compounds could be potential therapeutic agents for prevention and treatment of skin photoaging.