Salmonella typhimurium as a basis for a live oral Echinococcus granulosus vaccine
Salmonella typhimurium as a basis for a live oral Echinococcus granulosus vaccine
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DOI：
10.1016/s0264-410x(00)00197-3
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发表时间：
2000-10-15
期刊：
影响因子：
5.5
通讯作者：
Hormaeche, E
中科院分区：
文献类型：
--
作者：
Chabalgoity, JA;Moreno, M;Hormaeche, E
A live attenuated Salmonella typhimurium vaccine candidate, LVR01, was constructed by introducing a null deletion into the aroC gene of the parental canine S. typhimurium isolate, P228067. LVR01 was used to orally deliver to the canine immune system a fatty acid binding protein (FABP) from Echinococcus granulosus (EgDfl), as a fusion protein with fragment C (TetC) of tetanus toxin. Immunization studies demonstrated that live LVR01 is well tolerated by orally vaccinated dogs. There was no detectable shedding of the vaccine strain in the faeces 2 days after immunization. Humoral antibody responses were observed against Salmonella, TetC and EgDfl. Cellular responses were consistently detected against Salmonella and TetC. A cellular response against EgDfl was also seen in a proportion of the LVR01 vaccinated dogs. We propose S. typhimurium LVR01 as a carrier for recombinant antigens and a vector for the construction of multivalent oral vaccines for dogs. (C) 2000 Elsevier Science Ltd. All rights reserved.
