Reduction of Body Weight by Dietary Garlic Is Associated with an Increase in Uncoupling Protein mRNA Expression and Activation of AMP-Activated Protein Kinase in Diet-Induced Obese Mice
Reduction of Body Weight by Dietary Garlic Is Associated with an Increase in Uncoupling Protein mRNA Expression and Activation of AMP-Activated Protein Kinase in Diet-Induced Obese Mice
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DOI：
10.3945/jn.111.146050
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发表时间：
2011-11-01
影响因子：
4.2
通讯作者：
Kim, Yangha
中科院分区：
文献类型：
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作者：
Lee, Mak-Soon;Kim, In-Hwan;Kim, Yangha
本研究探讨大蒜对饮食诱导肥胖小鼠的减肥作用。雄性C57 BL/6 J小鼠喂食高脂饮食（45%脂肪）8周以诱导肥胖。随后，他们被喂食高脂肪对照饮食，高脂肪饮食补充2%，或5%大蒜（重量：重量），另外7周。饮食大蒜降低体重和各种白色脂肪组织沉积物的质量，也改善了高脂饮食引起的异常血浆和肝脏脂质分布。添加大蒜显著降低了白色脂肪组织（WAT）中成脂基因的mRNA水平。然而，大蒜的消费增加解偶联蛋白在棕色脂肪组织（BAT），肝脏，WAT和骨骼肌的mRNA的表达。大蒜处理的小鼠在6小时，4摄氏度的冷挑战期间保持比未处理的小鼠显著更高的体温，并且值得注意的是，在BAT，肝脏，WAT和骨骼肌中刺激AMP活化蛋白激酶（AMPK）活性。这些结果表明，大蒜的抗肥胖作用至少部分介导通过激活AMPK，增加产热，并减少参与脂肪形成的多个基因的表达。J.营养141：1947-1953，2011.
This study investigated the antiobesity effect of garlic in diet-induced obese mice. Male C57BL/6J mice were fed a high-fat diet (45% fat) for 8 wk to induce obesity. Subsequently, they were fed a high-fat control diet, high-fat diets supplemented with 2%, or 5% garlic (wt:wt) for another 7 wk. Dietary garlic reduced body weight and the mass of various white adipose tissue deposits and also ameliorated the high-fat diet-induced abnormal plasma and liver lipid profiles. Garlic supplementation significantly decreased the mRNA levels of adipogenic genes in white adipose tissues (WAT). However, consumption of garlic increased the expression of mRNA for uncoupling proteins in brown adipose tissue (BAT), liver, WAT, and skeletal muscle. Mice treated with garlic maintained a significantly higher body temperature than untreated mice during a 6-h, 4 degrees C cold challenge and, notably, AMP-activated protein kinase (AMPK) activity was stimulated in BAT, liver, WAT, and skeletal muscle. These results suggest that the antiobesity effects of garlic were at least partially mediated via activation of AMPK, increased thermogenesis, and decreased expression of multiple genes involved in adipogenesis. J. Nutr. 141: 1947-1953, 2011.