The Raman spectra of left-handed DNA oligomers incorporating adenine-thymine base pairs+.
The Raman spectra of left-handed DNA oligomers incorporating adenine-thymine base pairs+.
复制标题
掺入腺嘌呤-胸腺嘧啶碱基对的左手 DNA 寡聚物的拉曼光谱。
DOI：
10.1093/nar/12.14.5913
复制
发表时间：
1984
影响因子：
14.9
通讯作者：
ThomasJr,GJ
中科院分区：
文献类型：
--
作者：
Benevides,JM;Wang,AH;vanderMarel,GA;vanBoom,JH;Rich,A;ThomasJr,GJ
从[d（CGCATGCG）] 2和[d（m5 CGTAm 5CG）]2的单晶中获得了Reman光谱，这两种晶体都将A-T对掺入Z-DNA结构中，并且在寡核苷酸末端含有脱氧鸟苷的C2′-endo/syn构象异构体。与X射线结果的相关性允许对核苷构象体进行以下Reman分配：C3′-endo/syn C，623±1; C2′-endolsyn C，671±2; C end C，782±1; C2′endo/anti T，650±5和a. C3′-endolsyn A，729±1 cm。这些结果表明：（i）synG的670 cm-1谱线对C3-endo到Cr′-endo折叠的变化高度敏感，（ii）A的729 cm-1谱线既不受呋喃糖折叠的影响，也不受糖苷键方向的影响，（iii）A-T双螺旋的Reman光谱的1200-1500 cm-1区域被B-to-Z跃迁极大地改变。八聚体和六聚体在850-1700 cm-1区域的构象敏感拉曼频率被识别，并且通过将晶体溶解在H2O溶液中时发生的光谱变化来监测每种的Z到B跃迁。
Reman spectra were obtained from single crystals of [d(CGCATGCG)]2and [d(m5CGTAm5CG)]2, both of which incorporate A-T pairs into Z-DNA structures and contain C2′-endo/syn conformers of deoxyguanosine at the oligonucleotide ends. Correlation with x-ray results permits the following Reman assignments for nucleoside conformers: C3′-endo/syn C, 623±1; C2′-endolsyn C, 671±2; C end C, 782±1; C2′endo/anti T, 650±5 and a. 750; C3′-endolsyn A, 729±1 cm . These results show that (i) the 670 cm line ofsynG is highly 1 sensitive to the change from C3-endo to Cr′-endo pucker, (ii) the 729 cm−1line of A is affected neither by furanose pucker nor glycosidic bond orienta tion and (iii) the 1200-1500 cm−1region of the Reman spectrum of the A-T double helix is greatly altered by the B-to-Z transition. Conformation sensitive Raman frequencies in the 850–1700 cm−1region are identified for both octamer and hexamer, and the Z-to-B transition of each is monitored by spectral changes which occur upon dissolving the crystal in H2O solution.