Effect of hyperbaric oxygen and growth factors on rabbit ear ischemic ulcers.
Effect of hyperbaric oxygen and growth factors on rabbit ear ischemic ulcers.
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DOI：
10.1001/archsurg.1994.01420340057010
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发表时间：
1994-10
影响因子：
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通讯作者：
L. Zhao;J. Davidson;S. Wee;S. I. Roth;Thomas A. Mustoe, MD, FACS
中科院分区：
文献类型：
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作者：
L. Zhao;J. Davidson;S. Wee;S. I. Roth;Thomas A. Mustoe, MD, FACS
目的观察高压氧（HBO）、血小板源性生长因子-BB（PDGF-BB）和转化生长因子-β 1（TGF-β 1）对兔耳非收缩性皮肤溃疡标准化模型缺血所致创面愈合的影响。设计和干预在42只麻醉的年轻雌性新西兰白色兔的缺血耳中制造皮肤溃疡。对照组是在8只麻醉的年轻雌性新西兰白色兔的非缺血性耳中产生的溃疡。将PDGF-BB（5微克）、TGF-β 1（1微克）或单独的缓冲液应用于每个伤口，然后将其覆盖。有些组在第0天至第4天接受HBO治疗。在第7天收获伤口并进行组织学评价。主要观察指标：检测上皮再生量和肉芽组织生成量。评价伤口的糖胺聚糖和胶原含量。测量血管生成。结果：在缺血模型中，单独高压氧在第7天增加了约100%的新肉芽组织的产生，而没有显著影响新上皮的生长（P = 0.03）。相比之下，PDGF-BB和TGF-β 1在7天内均使新肉芽组织体积增加超过200%（P = .0001），并且对新上皮生长也具有统计学显著影响。然而，生长因子的HBO治疗产生了协同总逆转缺血产生的伤口愈合缺陷（P = 0.0001）。结论：PDGF-BB和TGF-β 1单独使用比HBO单独治疗更能促进缺血损伤创面愈合。然而，HBO与任一种生长因子的组合具有协同效应，完全逆转由缺血产生的缺陷。
OBJECTIVE To test the influence of hyperbaric oxygen (HBO), platelet-derived growth factor-BB (PDGF-BB), and transforming growth factor-beta 1 (TGF-beta 1) on the deficit in wound healing produced by ischemia in a noncontractive dermal ulcer standardized model in the rabbit ear. DESIGN AND INTERVENTIONS Dermal ulcers were created in the ischemic ears of 42 anesthetized young female New Zealand white rabbits. The controls were ulcers created in nonischemic ears of eight anesthetized young female New Zealand white rabbits. Either PDGF-BB (5 micrograms), TGF-beta 1 (1 microgram), or buffer alone was applied to each wound, which was then covered. Some groups were treated with HBO on days 0 through 4. Wounds were harvested on day 7 and were evaluated histologically. MAIN OUTCOME MEASURES The amount of epithelial regrowth and granulation tissue production were measured. The wounds were evaluated for glycosaminoglycan and collagen content. Angiogenesis was measured. RESULTS Hyperbaric oxygen alone, in the ischemic model, increased the production of new granulation tissue by approximately 100% at 7 days without significantly affecting new epithelial growth (P = .03). In contrast, PDGF-BB and TGF-beta 1 each increased the new granulation tissue volume by greater than 200% in 7 days (P = .0001) and also had a statistically significant effect on new epithelial growth. However, the addition of growth factors to HBO treatment produced a synergistic total reversal of the wound-healing deficit produced by ischemia (P = .0001). CONCLUSIONS Both PDGF-BB and TGF-beta 1 alone are more effective than HBO treatment by itself in accelerating the impaired wound healing produced by ischemia. However, the combination of HBO with either of the growth factors has a synergistic effect that totally reverses the deficit produced by ischemia.