PROTEIN DEGRADATION IN ISOLATED RAT HEPATOCYTES IS INHIBITED BY AMMONIA
PROTEIN DEGRADATION IN ISOLATED RAT HEPATOCYTES IS INHIBITED BY AMMONIA
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DOI：
10.1016/s0006-291x(75)80292-0
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
SEGLEN, O
中科院分区：
文献类型：
--
作者：
SEGLEN, O
新鲜分离的大鼠肝细胞，通过胶原酶灌注制备，内源性蛋白水解率高，不受氨基酸（作为生理混合物添加）的影响。然而，氨是氨基酸分解代谢的天然产物，通过从蛋白质释放酸溶性放射性或氮（氨基酸+尿素）来测量，可以抑制蛋白质降解。放射性氨基酸与蛋白质的结合不受影响。因此，氨似乎以一种选择性的方式抑制内源性肝蛋白降解，提供了一个终产物抑制的调节回路。
Freshly isolated rat hepatocytes, prepared by collagenase perfusion, exhibit a high rate of endogenous proteolysis which is unaffected by amino acids (added as a physiological mixture). However,ammonia, a natural product of amino acid catabolism, inhibits protein degradation as measured by the release from protein of either acid-soluble radioactivity or nitrogen (amino acids + urea). Incorporation of radioactive amino acids into protein is not affected. Thus, ammonia appears to inhibit endogenous hepatic protein degradation in a selective manner, providing a regulatory circuit of end-product inhibition.