Cell density modulates the metastatic aggressiveness of a mouse colon cancer cell line, colon 26
Cell density modulates the metastatic aggressiveness of a mouse colon cancer cell line, colon 26
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DOI：
10.1159/000082929
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发表时间：
2004-01-01
期刊：
影响因子：
3.5
通讯作者：
Asao, T
中科院分区：
文献类型：
--
作者：
Kuwano, H;Miyazaki, T;Asao, T
目的：尽管培养细胞中的细胞密度已经证明了细胞动力学性质的几种改变，但尚未研究不同细胞密度下癌细胞系转移侵袭性的变化。研究方法：在本研究中，我们通过评估肺转移的数量，研究了改变注射到BALB/c小鼠尾静脉中的培养癌细胞（colon 26和B16-F10）的细胞密度对转移活性的影响，并基于侵袭试验和细胞粘附试验的结果，讨论了这种现象的可能机制。结果如下：结肠26号高密度组的转移结节数明显高于低密度组（p < 0.005），而B16-F10组未见此现象。接下来，我们通过将环境改变为低密度组和高密度组中的细胞的相反条件来进行相同的实验，结果显示转移活性在高密度组中总是更高。此外，尽管在高密度和低密度组之间没有观察到关于侵袭活性的差异，但粘附测定显示粘附细胞率的差异在高密度组中显著更高，特别是在与人脐静脉内皮细胞（HUVEC）共培养后的早期（p < 0.05）。结论：在一些细胞类型中，转移活性可以通过在相对短的时间内改变环境（例如细胞密度）而改变和逆转。结果表明，癌细胞的表观遗传学改变在恶性潜能中起一定作用。版权所有（C）2004 S. Karger AG，巴塞尔。
Objective: Although cell density in cultured cells has demonstrated several alterations in the nature of cell kinetics, the changes in the metastatic aggressiveness of cancer lines under different cell densities have not yet been studied. Methods: In the current study, we investigated the influence of changing the cell density of cultured cancer cells ( colon 26 and B16-F10) injected into the tail vein in BALB/c mice on the metastatic activity by evaluating the number of lung metastases, and the possible mechanisms of this phenomenon were discussed based on the basis of the results of an invasion assay and a cell adhesion assay. Results: The number of metastatic nodules was significantly higher in the high-density group than in the low one in colon 26 ( p < 0.005), however, this phenomenon was not seen in B16-F10. Next, we performed the same experiment by changing the environment to the opposite conditions for the cells in the low- and high-density groups, and the results showed the metastatic activities to be always higher in the high-density group. Moreover, although no difference was seen regarding the invasive activity between the high- and low-density groups, an adhesion assay showed the difference in the adhesion cell rate to be significantly higher in the high- density group especially in early period after coculture with human umbilical vein endothelial cells (HUVEC) ( p < 0.05). Conclusion: In some cell types, the metastatic activity could be altered and reversed by changing the environment, such as the cell density, during a relatively short period. As a result, the epigenetic changes of cancer cells are thus suggested to play a certain role in the malignant potentiality. Copyright (C) 2004 S. Karger AG, Basel.