Protein dielectrophoresis and the link to dielectric properties.
Protein dielectrophoresis and the link to dielectric properties.
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作者：
Camacho-Alanis F;Ros A
蛋白质介电电泳（DEP）在生物技术和制药领域的应用越来越受到人们的关注。然而，蛋白质的DEP行为仍然没有得到很好的理解，这对于成功的蛋白质操纵是重要的。在本文中，我们阐明了在介电谱（DS）和电化学阻抗谱（EIS）中获得的信息，以及这些技术如何可能对未来的蛋白质DEP操纵的重要性。EIS和DS可用于确定蛋白质的介电性质，预测其DEP行为。本文讨论了电化学阻抗谱和动态谱的基本原理，并以蛋白质电泳为例，介绍了电化学阻抗谱和动态谱的应用。执行DS测量以及潜在的设计，将EIS和DS测量DEP实验的挑战也进行了讨论。
There is a growing interest in protein dielectrophoresis (DEP) for biotechnological and pharmaceutical applications. However, the DEP behavior of proteins is still not well understood which is important for successful protein manipulation. In this paper, we elucidate the information gained in dielectric spectroscopy (DS) and electrochemical impedance spectroscopy (EIS) and how these techniques may be of importance for future protein DEP manipulation. EIS and DS can be used to determine the dielectric properties of proteins predicting their DEP behavior. Basic principles of EIS and DS are discussed and related to protein DEP through examples from previous studies. Challenges of performing DS measurements as well as potential designs to incorporate EIS and DS measurements in DEP experiments are also discussed.