Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B
Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B
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DOI：
10.1016/j.fct.2007.06.034
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Zhao, Li-Bin
中科院分区：
文献类型：
--
作者：
Zhao, Guang-Rong;Zhang, Heng-Ming;Zhao, Li-Bin
丹参素（3-（3，4-dihydroxyphenyl）lactic acid，3-（3，4-dihydroxyphenyl）lactic acid）和丹参素B（salviolate acid，B）是从丹参中分离得到的两种天然酚酸类咖啡酸衍生物，是治疗多种心血管疾病的常用中药。为了更好地了解它们的生物学功能，沿着维生素C，评价了丹参素和丹参素酸B的体外自由基清除和抗氧化活性。丹参素和丹参酸B对羟自由基（HO中心点）、超氧阴离子自由基（O-2（中心点-））、DPPH自由基和ABTS自由基的清除能力均高于维生素C。丹参素和丹参素酸B的铁螯合活性和过氧化氢清除活性均弱于维生素C。以维生素C当量（VCEAC）表示，ABTS自由基法测定的相对VCEAC值（mg/100 ml）为丹参素B（18.59）>丹参素（12.89）>维生素C（10.00）。抗氧化活性与其对过氧化氢诱导的人静脉血管内皮细胞损伤的保护作用有关。构效关系分析表明，丹参素与咖啡酸的缩合和共轭作用对抗氧化活性起重要作用。结果表明丹参素和丹参酸B均为有效的自由基清除剂和抗氧化剂，且丹参酸B上级丹参素。它们的自由基清除和抗氧化性能可能在食品和保健工业中具有潜在的应用。(c)2007爱思唯尔有限公司保留所有权利。
Danshensu (3-(3,4-dihydroxyphenyl) lactic acid) and salvianolic acid B, two natural phenolic acids of caffeic acid derivatives isolated from Salvia miltiorrhiza root of the most widely used traditional Chinese medicine for the treatment of various cardiovascular diseases, have been reported to have potential protective effects from oxidative injury. To better understand their biological functions, the in vitro radical scavenging and antioxidant activities of danshensu and salvianolic acid B were evaluated along with vitamin C. Both danshensu and salvianolic acid B exhibited higher scavenging activities against free hydroxyl radicals (HO center dot), superoxide anion radicals (O-2(center dot-)), 1, 1 diphenyl-2-picryl-hydrazyl (DPPH) radicals and 2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals than vitamin C. In contrary, danshensu and salvianolic acid B showed weaker iron chelating and hydrogen peroxide (H2O2) scavenging activities than vitamin C. As expressed as vitamin C equivalent capacity (VCEAC), the relative VCEAC values (mg/100 ml) were in the order of salvianolic acid B (18.59) > danshensu (12.89) > vitamin C (10.00) by ABTS radical assay. The protective efficiencies against hydrogen peroxide induced human vein vascular endothelial cell damage were correlated with their antioxidant activities. Analysis of structure-activity relationship of these two compounds showed that the condensation and conjugation of danshensu and caffeic acid appears important for antioxidant activity. These results indicated that danshensu and salvianolic acid B are efficient radical scavengers and antioxidants, and salvianolic acid B is superior to danshensu. Their radical scavenging and antioxidant properties might have potential applications in food and healthcare industry. (c) 2007 Elsevier Ltd. All rights reserved.