Changes in the biosynthesis and catabolism of polyamines in isolated plastids during chloroplast photodevelopment
Changes in the biosynthesis and catabolism of polyamines in isolated plastids during chloroplast photodevelopment
复制标题
DOI：
10.1016/1011-1344(95)07240-3
复制
发表时间：
1996-04-01
影响因子：
5.4
通讯作者：
Kotzabasis, K
中科院分区：
文献类型：
--
作者：
Andreadakis, A;Kotzabasis, K
分离到的黄体、叶绿体和叶绿体中含有大量的多胺(PAS)、二胺氧化酶(DAO)活性以及通过鸟氨酸(ODC)和精氨酸(ADC*)形成的腐胺。分离的黄体含有大量的PAS(腐胺(Put)、亚精胺(Spd)和精胺(Spm))，主要的PA生物合成酶ODC和ADC*的活性很高，而PA主要分解酶DAO的活性很低。在叶绿体向叶绿体的光发育过程中，叶绿体内的PAS、ODC和ADC*发生了两步下降：第一步非常快，第二步在长时间光照后更强烈。讨论了这两步与原叶绿素(PChlide)到叶绿素(Chlide)的光转化和光合作用器官的发育的关系。我们的实验表明，在光照9h后，黄体中几乎为零的DAO活性迅速增加，并出现最大值，而细胞内DAO活性几乎保持不变。叶绿体光发育过程中的所有可塑性活性(ODC、ADC*、DAO)和PAS水平(Put、Spd、Spm)都与相应的胞内值相关，并讨论了它们之间的差异。
Polyamines (PAs), diamine oxidase (DAO) activity and putrescine formation via ornithine (ornithine decarboxylase, ODC) and arginine (arginine decarboxylase and arginase pathway, ADC*) were found in isolated etioplasts, chloroplasts and the intermediate plastids of chloroplast photodevelopment.The isolated etioplasts contain large amounts of PAs (putrescine (Put), spermidine (Spd), spermine (Spm)), and high activities of the main PA biosynthetic enzymes ODC and ADC*, combined with very low activity of the main PA catabolic enzyme DAO, were found. During the photodevelopment of etioplasts to chloroplasts, a two-step decrease of intraplastidic PAs, ODC and ADC* takes place: the first very fast and the second stronger after a very long exposure to light.The correlation of these two steps with the protochlorophyllide (PChlide) to chlorophyllide (Chlide) photoconversion and the development of the photosynthetic apparatus is discussed. Our experiments indicate that the almost zero DAO activity in etioplasts increases rapidly and shows a maximum after exposure to light for 9 h, whereas the intracellular DAO activity remains almost unchanged. All the plastidic activities (ODC, ADC*, DAO) and the level of PAs (Put, Spd, Spm) during the photodevelopment of chloroplasts are correlated with the corresponding intracellular values and the differences are discussed.