Tissue dynamics of CD8 lymphocytes that suppress viral replication in cats infected neonatally with feline immunodeficiency virus.
Tissue dynamics of CD8 lymphocytes that suppress viral replication in cats infected neonatally with feline immunodeficiency virus.
复制标题
CD8 淋巴细胞的组织动力学抑制新生儿感染猫免疫缺陷病毒的猫的病毒复制。
DOI：
10.1086/323076
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Johnson,CM
中科院分区：
文献类型：
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作者：
Crawford,PC;Papadi,GP;Levy,JK;Benson,NA;Mergia,A;Johnson,CM
本研究的目的是确定抑制猫免疫缺陷病毒（FIV）复制的CD8淋巴细胞的组织分布和抗病毒活性。在新生儿接种后，在血液、胸腺和周围淋巴结中连续测量细胞相关的FIV载量、CD8α+β低细胞和CD8细胞介导的FIV抑制。在6 - 10周期间，感染猫胸腺和血液中CD8α+β低细胞的相对数量增加，而CD8α+β高细胞的相对数量减少。到12-16周时，由于所有CD8β亚群的绝对扩增，淋巴结肿大。体外CD8细胞介导的FIV抑制强度与胸腺和血液中的病毒载量呈负相关，而CD8α+β低细胞含量与此无关。因此，在新生儿接种FIV后，CD8α+β低细胞首先占据胸腺和血液，其中CD8细胞介导的强抗病毒活性与多种淋巴组织中病毒载量的减少有关
The purpose of this study was to determine the tissue distribution and antiviral activity of the CD8 lymphocytes that suppress the replication of feline immunodeficiency virus (FIV). Cell-associated FIV load, CD8α+βlowcells, and CD8 cell–mediated suppression of FIV were measured serially in the blood, thymus, and peripheral lymph nodes after neonatal inoculation. Between 6 and 10 weeks, relative numbers of CD8α+βlowcells increased, whereas CD8α+βhighcells declined in the thymus and blood of infected cats. By 12–16 weeks, the lymph nodes were enlarged because of an absolute expansion of all CD8β subpopulations. The strength of CD8 cell–mediated FIV suppression in vitro, but not CD8α+βlowcell content, was correlated inversely with virus load in the thymus and blood. Thus, after neonatal FIV inoculation, CD8α+βlowcells first occupy the thymus and blood, where strong CD8 cell–mediated antiviral activity is linked to reduced virus load in multiple lymphoid tissues