Fluence and wavelength requirements for arabidopsis CAB gene induction by different phytochromes
Fluence and wavelength requirements for arabidopsis CAB gene induction by different phytochromes
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DOI：
10.1104/pp.115.4.1533
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发表时间：
1997-12-01
期刊：
影响因子：
7.4
通讯作者：
Furuya, M
中科院分区：
文献类型：
--
作者：
Hamazato, F;Shinomura, T;Furuya, M
研究了不同光敏色素在拟南芥叶绿素a/b结合蛋白基因（CAB）光诱导中的作用。将野生型、光敏色素A（PhyA）无效突变体（phyA）、光敏色素B（PhyB）无效突变体（phyB）和phyA/phyB双突变体的黄化幼苗暴露于单色光以解决不同光敏色素诱导CA B的能量密度和波长要求的问题。在野生型和phyB突变体中，PhyA光不可逆地诱导CAB表达后，用非常低的能量密度的光照射350至750 nm。相比之下，使用phyA突变体，PhyB光可逆地诱导CAB表达与低通量红光。红光诱导PhyA和PhyB的阈值通量分别为10 nmol m（-2）和10 μ mol m（-2）。此外，CAB表达的光可逆诱导与低通量红光的phyA/phyB双突变体，揭示了另一种光敏色素（S）（PhyX）调节CAB表达的方式类似于PhyB。这些数据表明，植物利用不同的光敏色素来感知不同波长和通量的光，并开始解释植物如何对环境中不断变化的光做出如此微妙的反应。
The roles of different phytochromes have been investigated in the photoinduction of several chlorophyll a/b-binding protein genes (CAB) of Arabidopsis thaliana. Etiolated seedlings of the wild type, a phytochrome A (PhyA) null mutant (phyA), a phytochrome B (PhyB) null mutant (phyB), and a phyA/phyB double mutant were exposed to monochromatic light to address the questions of the fluence and wavelength requirements for CAB induction by different phytochromes. In the wild type and the phyB mutant, PhyA photoirreversibly induced CAB expression upon irradiation with very-low-fluence light of 350 to 750 nm. In contrast, using the phyA mutant, PhyB photoreversibly induced CAB expression with low-fluence red light. The threshold fluences of red light for PhyA- and PhyB-specific induction were about 10 nmol m(-2) and 10 mu mol m(-2) respectively. In addition, CAB expression was photoreversibly induced with low-fluence red light in the phyA/phyB double mutant, revealing that another phytochrome(s) (PhyX) regulated CAB expression in a manner similar to PhyB. These data suggest that plants utilize different phytochromes to perceive light of varying wavelengths and fluence, and begin to explain how plants respond so exquisitely to changing light in their environment.