Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells.
Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells.
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DOI：
10.1016/j.xpro.2020.100031
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发表时间：
2020-06-19
期刊：
影响因子：
--
通讯作者：
Dalton S
中科院分区：
文献类型：
--
作者：
Chen L;Dalton S
血管化对于器官的稳态和功能至关重要，但促进血管再生的基于细胞的技术是有限的。该方案描述了产生人多能干细胞(HPSC)来源的间皮细胞系血管前体细胞的步骤。这项技术在培养血管细胞方面有几个优势。首先也是最重要的是，MesoT细胞是多能祖细胞，可以生成平滑肌细胞和内皮细胞。因此，MesoT细胞在组织修复、组织工程和实验室培养器官的血管形成中具有潜在的用途。有关本议定书的使用和执行的完整细节，请参阅。人多能干细胞(HPSCs)分化为血管前体细胞(MesoT细胞)在胚胎心脏中相当于体内的血管前体细胞hPSC来源的MesoT细胞是多能的，来源于间皮细胞系人类MesoT细胞在再生医学中具有潜在的应用价值血管形成对器官的动态平衡和功能至关重要，但基于细胞的促进血管再生的技术有限。该方案描述了产生人多能干细胞(HPSC)来源的间皮细胞系血管前体细胞的步骤。这项技术在培养血管细胞方面有几个优势。首先也是最重要的是，MesoT细胞是多能祖细胞，可以生成平滑肌细胞和内皮细胞。因此，MesoT细胞在组织修复、组织工程和实验室培养器官的血管形成中具有潜在的用途。
Vascularization is critical for organ homeostasis and function, but cell-based technologies that promote vascular regeneration are limited. This protocol describes steps to generate human pluripotent stem cell (hPSC)-derived vascular progenitors of the mesothelium lineage. This technology has several advantages for the generation of vascular cells. First and foremost, MesoT cells are multipotent progenitors that can generate smooth muscle cells and endothelial cells. MesoT cells therefore have potential utility in tissue repair, tissue engineering, and in vascularization of laboratory grown organs. For complete details on the use and execution of this protocol, please refer to. Generation of vascular progenitors (MesoT) from human pluripotent cells (hPSCs) MesoT cells are equivalent to in vivo vascular progenitor cells in the embryonic heart hPSC-derived MesoT cells are multipotent and derived from the mesothelium lineage Human MesoT cells have potential utility in regenerative medicine Vascularization is critical for organ homeostasis and function, but cell-based technologies that promote vascular regeneration are limited. This protocol describes steps to generate human pluripotent stem cell (hPSC)-derived vascular progenitors of the mesothelium lineage. This technology has several advantages for the generation of vascular cells. First and foremost, MesoT cells are multipotent progenitors that can generate smooth muscle cells and endothelial cells. MesoT cells therefore have potential utility in tissue repair, tissue engineering, and in vascularization of laboratory grown organs.
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影响因子：
4.6
作者：
Sumi, Tomoyuki;Tsuneyoshi, Norihiro;Suemori, Hirofumi
通讯作者：
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影响因子：
8.8
作者：
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影响因子：
13.5
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通讯作者：
Duncan, Stephen A.
影响因子：
64.8
作者：
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通讯作者：
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影响因子：
14.8
作者：
Menendez, Laura;Kulik, Michael J.;Dalton, Stephen
通讯作者：
Dalton, Stephen