GENETIC DIVERSITY OF CHINESE THIN-SKINNED MELON CULTIVARS (CUCUMIS MELO L.) BASED ON SIMPLE SEQUENCE REPEAT MARKERS**
GENETIC DIVERSITY OF CHINESE THIN-SKINNED MELON CULTIVARS (CUCUMIS MELO L.) BASED ON SIMPLE SEQUENCE REPEAT MARKERS**
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DOI：
10.17660/actahortic.2007.763.22
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发表时间：
2007-11
期刊：
影响因子：
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通讯作者：
Y. Sheng;F. Luan;K. Chen;Xi-Wen Cui;J. Staub
中科院分区：
文献类型：
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作者：
Y. Sheng;F. Luan;K. Chen;Xi-Wen Cui;J. Staub
Genetic diversity among 46 Chinese thin-skinned melon cultivars (Cucuinis melo L. 2n=2x=24) of diverse origin was assessed using 50 simple sequence repeat (SSR) markers. Thirty of 48 primers amplified polymorphisms, where 179 bands (-4 bands per SSR primer) defined the genotypes studied. Forty-six accessions were partitioned into nine groups using multidimensional scaling (MDS) and cluster tree analysis. Twenty-one of the 46 accessions were selected from these nine groups for repeated analyses. There was no difference between MDS and cluster analysis using these nine and all accessions (46) indicating that both multivariate approaches were effective in describing genetic relationships among the accessions examined. Moreover, the data indicated that genetic diversity of the Chinese thin-skinned melon cultivars examined could be enhanced by the introduction of genetic variation from Chinese accessions of different origins. Additionally, it would be advantageous to acquire more accessions from geographically varied regions to ensure the retention of existing genetic diversity in China. INTRODUCTION Melon (Cucumis mc'/o L.; 2n2x=24, Cucurbitaceae) is a morphologically diverse, outcrossing horticultural crop of broad economic importance (Kirkbride, 1993). Monger and Robinson's (1991) revision of melon taxonomic and horticultural groups includes: (I) C. inc/U agrestis Naud. (wild melon): (2) C. melo /7exuosus Group Flexuosus Naud. (snake melon): (3) C. melo conomon Group Conomon Mak. (pickling melon, Chinese white cucumber) (4) C. me/U canta/upensis Group Cantalupensis Naud. (cantaloupe or muskmelon): (5) C. inelo inodorus Group Inodorus Naud. (winter melons, honeydew, Casaba): (6) C. me/U me/ochito (mango melon) and dudaim Naud. (Queen's pocket melon), and: (7) C. inc/u ,no,nordica (phootor snap melon) (M liki et al., 2001). This taxonomic classification has been used in various diversity analyses ol'melon (Mliki et al., 2001: Nakata et al.. 2005). Wild melons (C. me/u subsp. agrestis) develop slender vines and small, inedible fruit, and frequently commercialized melon belong to well-recognized horticultural groups. Fruits of Momordica and Conomon types are cultivated in South and East Asia and develop smooth-skinned fruits which have low sugar content. Most Chinese melons develop thick-skinned fruit. Hcilongjiang province has a long history of melon cultivation, where several morphotypes are grown under different temperature and day-length conditions. It has become important to broaden and enhance the melon types grown in this province. Molecular markers have been used effectively in map construction and genetic diversity analyses (Staub et a].. 1996a: Bretting and Widrlechner, 1995). Genetic variation at marker loci (e.g., RFLP, AFLP and RAPD) in melon have allowed for cultivar discrimination. Staub et al. (1999, 2001) successfully used RAPID marker loci to discriminate genetically narrow based accessions, primarily in 'Galia' and*Pie] de Sapo' market classes. Mo-Suk et al. (1999) used eight polymorphic RAPD bands to differentiate 52 Korean landraces and lines into two distinct groups (Ferguson et a]., 2004). More Proc. XXVI! IHC-S7 Stnict. and Funct. (jenomics of fort. Plants Ed.-in-Chief: R. Drew 169 Acts HorS. 763, ISHS 2007
