Genome-Scale Transcript Analyses of Human Neutrophils.
Genome-Scale Transcript Analyses of Human Neutrophils.
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人类中性粒细胞的基因组规模转录分析。
DOI：
10.1007/978-1-0716-0154-9_21
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
DeLeo,FrankR
中科院分区：
文献类型：
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作者：
Kobayashi,ScottD;Porter,AdelineR;Anzick,SarahL;Sturdevant,DanE;DeLeo,FrankR
Transcriptome analyses of unicellular and multicellular organisms have changed fundamental understanding of biological and pathological processes across multiple scientific disciplines. Over the past 15 years, studies of polymorphonuclear leukocyte (PMN or neutrophil) gene expression on a global scale have provided new insight into the molecular processes that promote resolution of infections in humans. Herein we present methods to analyze gene expression in human neutrophils using Affymetrix oligonucleotide microarrays and next-generation sequencing. Notably, the procedures utilize commercially available reagents and materials and thus represent a standardized approach for evaluating PMN transcript levels.
