Central effects of a local inflammation in three commonly used mouse strains with a different anxious phenotype
Central effects of a local inflammation in three commonly used mouse strains with a different anxious phenotype
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DOI：
10.1016/j.bbr.2011.05.011
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发表时间：
2011-10-10
影响因子：
2.7
通讯作者：
Blom, Joan M. C.
中科院分区：
文献类型：
--
作者：
Benatti, Cristina;Alboni, Silvia;Blom, Joan M. C.
与人类一样，啮齿类动物的遗传背景可能会影响一系列特殊的行为特征，例如对疼痛和压力或焦虑相关行为的敏感性。因此，我们检验了具有不同遗传背景的小鼠[远交（CD 1），近交（CD 2）]（C57 BL/6 J）和杂交（B6 C3 F1）成年雄性小鼠]显示出对疼痛、应激和焦虑相关行为的反应性改变。我们证明，B6 C3 F1小鼠相对于C57 BL/6 J或CD 1动物显示出更焦虑的表型，当在开放场地和高架十字迷宫中测试时，后者是较不焦虑的品系。在对辐射热源的热敏感性方面，没有观察到菌株之间的差异。然后用炎性剂完全弗氏佐剂（CFA）的足底下注射处理小鼠，24小时后，它们相对于盐水暴露的动物是痛觉过敏的，与品系无关。然后，我们测量了品系内差异和CFA诱导的品系间对各种基因表达的影响，这些基因在疼痛和焦虑中具有公认的作用：小鼠丘脑，海马和下丘脑中的BDNF，IL-6，IL-1 β IL-18和NMDA受体亚基。与远交系CD 1和C57 BL/6 J近交系小鼠相比，在B6 C3 F1杂交小鼠中观察到的更多焦虑表型显示海马和下丘脑中BDNF mRNA水平较低。CFA导致评估的靶点的中心基因表达普遍降低，特别是在CD 1小鼠中，而BDNF下丘脑下调在评估的所有三种品系中作为CFA的共同效应而突出。(C)2011 Elsevier B. V.保留所有权利。
As in humans, genetic background in rodents may influence a peculiar set of behavioural traits such as sensitivity to pain and stressors or anxiety-related behaviours. Therefore, we tested the hypothesis that mice with different genetic backgrounds [outbred (CD1), inbred (C57BL/6J) and hybrid (B6C3F1) adult male mice] display altered reactivity to pain, stress and anxiety related behaviours.We demonstrated that B6C3F1 mice displayed the more anxious phenotype with respect to C57BL/6J or CD1 animals, with the latter being the less anxious strain when tested in an open field and on an elevated plus maze. No difference was observed across strains in thermal sensitivity to a radiant heat source. Mice were then treated with a sub-plantar injection of the inflammatory agent Complete Freund's Adjuvant (CFA), 24 h later they were hyperalgesic with respect to saline exposed animals, irrespective of strain. We then measured intra-strain differences and CFA-induced inter-strain effects on the expression of various genes with a recognized role in pain and anxiety: BDNF, IL-6, IL-1 beta IL-18 and NMDA receptor subunits in the mouse thalamus, hippocampus and hypothalamus. The more anxious phenotype observed in B6C3F1 hybrid mice displayed lower levels of BDNF mRNA in the hippocampus and hypothalamus when compared to outbred CD1 and C57BL/6J inbred mice. CFA led to a general decrease in central gene expression of the evaluated targets especially in CD1 mice, while BDNF hypothalamic downregulation stands out as a common effect of CFA in all three strains evaluated. (C) 2011 Elsevier B.V. All rights reserved.