Xsox17 alpha and -beta mediate endoderm formation in Xenopus
Xsox17 alpha and -beta mediate endoderm formation in Xenopus
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DOI：
10.1016/s0092-8674(00)80423-7
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发表时间：
1997-10-31
期刊：
影响因子：
64.5
通讯作者：
Woodland, HR
中科院分区：
文献类型：
--
作者：
Hudson, C;Clements, D;Woodland, HR
我们已经分离到两个非洲爪哇近缘的小鼠Sox17在原肠胚假定内胚层中表达。激活素可以诱导动物帽子中Xsox17α和β蛋白的表达，但成纤维细胞生长因子不能诱导其表达。这些基因在动物帽中的异位表达诱导内胚层标记物的表达；这种诱导作用被Xsox17βHMG结构域与果蝇增强抑制器域的融合所阻断，激活素诱导内胚层标记物的表达，以及内胚层标记物在整个胚胎和分离的植物极中的表达。这些实验以及mRNAs对胚胎表型的影响表明，Xsox17基因介导了激活素诱导的动物帽子内胚层分化途径，并参与了胚胎正常的内胚层分化。
We have isolated two Xenopus relatives of murine Sox17 expressed in gastrula presumptive endoderm. Xsox17 alpha and -beta expression can be induced in animal caps by activin, but not by FGF. Ectopic expression of these genes in animal caps induces the expression of endoderm markers; this induction is blocked by overexpression of a fusion of the Xsox17 beta HMG domain to the Drosophila Engrailed repressor domain, as is induction of endoderm markers by activin and the expression of endodermal markers in whole embryos and isolated vegetal poles. These experiments, as well as the effects of the mRNAs on embryo phenotypes, suggest that the Xsox17 genes mediate an activin-induced endoderm differentiation pathway in animal caps and are involved in normal endoderm differentiation in embryos.