Genetic diversity and characterization of heavy metal-resistant-endophytic bacteria from two copper-tolerant plant species on copper mine wasteland.
Genetic diversity and characterization of heavy metal-resistant-endophytic bacteria from two copper-tolerant plant species on copper mine wasteland.
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DOI：
10.1016/j.biortech.2009.08.011
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发表时间：
2010
影响因子：
11.4
通讯作者：
Leni Sun;YAN-FENG Zhang;Linyan He;Zhao-jin Chen;Qing-ya Wang;Meng Qian;X. Sheng
中科院分区：
文献类型：
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作者：
Leni Sun;YAN-FENG Zhang;Linyan He;Zhao-jin Chen;Qing-ya Wang;Meng Qian;X. Sheng
通过使用培养和独立培养技术评估了耐铜物种香薷和鸭跖草内生细菌的多样性。基于16S rDNA序列的系统发育分析表明，铜抗性分离株属于三个主要系统发育类群：厚壁菌门、放线菌门和变形菌门。铜抗性菌株中以芽孢杆菌和不动杆菌为主。对两种植物根部的 16S rDNA 克隆文库进行的序列分析揭示了 Alpha-、Beta-、Gamma-Proteobacteria、Firmicutes、Actinobacteria 和 Bacteroidetes 的序列。 γ-变形菌门在两个 16S rDNA 克隆文库中占主导地位。分离出 32 种内生细菌，并对其重金属抗性和植物生长促进因子的产生进行了表征。在涉及在含有 4mgkg−1 Cu 的蛭石中生长的油菜植物的实验中，发现与未接种的对照相比，接种分离物可增加根部干重（范围从 132% 到 155%）和地上组织干重（范围从 71% 到 83%）。与未接种的对照相比，在铜污染基质中培养的接种油菜植株地上组织铜含量增加了 63% 至 125%。
The diversity of endophytic bacteria from the copper-tolerant species Elsholtzia splendens and Commelina communis was evaluated by using cultivation and cultivation-independent techniques. Phylogenetic analysis based on 16S rDNA sequences showed that the Cu-resistant isolates belonged to three major phylogenetic groups: Firmicutes, Actinobacteria and Proteobacteria. Bacillus and Acinetobacter were predominant among the Cu-resistant isolates. Sequence analysis from the 16S rDNA clone libraries of the two plant roots revealed sequences of Alpha-, Beta-, Gamma-Proteobacteria, Firmicutes,Actinobacteria and Bacteroidetes. Gammaproteobacteria was predominant in the two 16S rDNA clone libraries. Thirty-two endophytic bacteria were isolated and characterized with respect to heavy metal resistance and production of plant growth-promoting factors. In experiments involving rape plants grown in vermiculite containing 4mgkg−1of Cu, inoculation with the isolates was found to increase the dry weights of roots (ranging from 132% to 155%) and above-ground tissues (ranging from 71% to 83%) compared to the uninoculated control. Increase in above-ground tissue Cu content varied from 63% to 125% in inoculated-rape plants cultivated in Cu-contaminated substrate compared to the uninoculated control.