Visualizing viral assemblies in a nanoscale biosphere
Visualizing viral assemblies in a nanoscale biosphere
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DOI：
10.1039/c2lc41008g
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Kelly, Deborah F.
中科院分区：
文献类型：
--
作者：
Gilmore, Brian L.;Showalter, Shannon P.;Kelly, Deborah F.
我们提出了一种新的微流控平台，以高分辨率检查生物组件。我们设计了一个功能化的腔室，作为一个“纳米生物圈”，以捕获和保持轮状病毒双层颗粒（DLP）在液体环境中。该室可以插入到透射电子显微镜的柱中，同时与真空系统完全隔离。这种配置使我们能够在一个独立的容器内以纳米分辨率确定生物复合物的结构。DLP的图像用于计算溶液中大分子的第一个3D视图。我们把这种新的流体可视化技术称为原位分子显微镜。
We present a novel microfluidic platform to examine biological assemblies at high-resolution. We have engineered a functionalized chamber that serves as a "nanoscale biosphere'' to capture and maintain rotavirus double-layered particles (DLPs) in a liquid environment. The chamber can be inserted into the column of a transmission electron microscope while being completely isolated from the vacuum system. This configuration allowed us to determine the structure of biological complexes at nanometer-resolution within a self-contained vessel. Images of DLPs were used to calculate the first 3D view of macromolecules in solution. We refer to this new fluidic visualization technology as in situ molecular microscopy.