Surface modification strategy based on the conjugation of NaYF4:5%Eu luminescent nanoprobe with organic aromatic compounds for application in bioimaging assays
Surface modification strategy based on the conjugation of NaYF4:5%Eu luminescent nanoprobe with organic aromatic compounds for application in bioimaging assays
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DOI：
10.1007/s11051-018-4422-0
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发表时间：
2019-01
影响因子：
2.5
通讯作者：
Rodrigo Silva Viana;Luciana Amaral Mascena Costa;A. N. Leal;Tyrslai M. Williams;Liquian Luan;Guanyun Zhang;Maodie Wang;A. Harmon;J. V. Anjos;R. Cueto;M. A. G. Filho;E. Falcão;M. Vicente;S. Júnior;J. Mathis
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文献类型：
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作者：
Rodrigo Silva Viana;Luciana Amaral Mascena Costa;A. N. Leal;Tyrslai M. Williams;Liquian Luan;Guanyun Zhang;Maodie Wang;A. Harmon;J. V. Anjos;R. Cueto;M. A. G. Filho;E. Falcão;M. Vicente;S. Júnior;J. Mathis
结肠癌是世界上最致命的疾病之一。由于它的内部位置，有必要获得更快和更有效的诊断工具来诊断这个器官部位。在这种背景下，我们研究了新型发光纳米探针(LNPs)的开发，作为检测这种疾病的替代诊断工具。本文研究的纳米颗粒是掺镧氟化钠(NaYF4：LN)，已被证明是很有希望作为研究装置的。然而，使用LNPs的重大问题是缺乏生物相容性和系统针对肿瘤区域的靶向性。避免这些问题的策略之一是增加颗粒的亲油性，用与生物系统高度相似的有机化合物来修饰颗粒的表面。在这项工作中，我们合成了六种用于生物成像技术的新材料，这些材料是通过将纳米NaYF4：5%Eu与有机芳香族化合物共价键合而得到的。用X射线衍射仪和透射电子显微镜对材料的结构和形貌进行了表征，确定了β-NaYF_4：5%Eu的晶相及其纳米尺寸(颗粒小于50 nm)。通过红外光谱分析和热重分析证实了偶联过程。激发光谱和发射光谱可以用来评价合成化合物的光学性质。当HT-29结肠癌细胞暴露在LNPs中时，这种相互作用和细胞摄取得到了证实，表明所开发的系统在生物成像过程中具有潜在的应用前景。
Colon cancer is one of the world’s most deadly diseases. Because of its internal location, it is necessary to obtain faster and more efficient diagnostic tools for this organ site. In this context, we studied the development of new luminescent nanoprobes (LNPs) as an alternative diagnostic apparatus for detecting this disease. The nanoparticles examined herein are lanthanide-doped sodium yttrium fluoride (NaYF4:Ln) and have shown to be promising as investigative devices. However, significant problems with the use of LNPs are the lack of biocompatibility and the targeting of the system to tumor regions. One of the strategies to bypass these problems is to increase of the particle lipophilicity modifying their surfaces with organic compounds that present high similarity to the biological system. In this work, we synthesized six new materials for use in bioimaging techniques obtained from the combination of nanoparticles of NaYF4:5%Eu with organic aromatic compounds covalently bonded. The materials were characterized structurally and morphologically using XRD and TEM, techniques, which showed the identification of the crystallographic phase β-NaYF4:5%Eu and its nanometric size (particles smaller than 50 nm). The conjugation process was confirmed by FT-IR spectra analysis and from the TGA profile. Excitation and emission spectra allowed the evaluation of the optical properties of the synthesized compounds. The interaction and cellular uptake was confirmed when HT-29 colon cancer cells were exposed to LNPs, indicating that the developed system has promising applications in bioimaging procedures.Graphical abstract