Orange emissive N,S-codoped carbon dots for label-free and sensitive fluorescence assay of vitamin B12
Orange emissive N,S-codoped carbon dots for label-free and sensitive fluorescence assay of vitamin B12
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用于维生素 B12 的无标记和灵敏荧光测定的橙色发射 N,S 共掺杂碳点
DOI：
10.1039/d1nj04706j
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发表时间：
2021
影响因子：
3.3
通讯作者：
Shengyuan Yang
中科院分区：
文献类型：
--
作者：
Pengfei Fan;Can Liu;Congcong Hu;Feifei Li;Xi Lin;Fubing Xiao;Hao Liang;Le Li;Shengyuan Yang
以邻苯二胺和硫脲为前驱体，通过水热法制备了具有强橙色荧光的氮硫共掺杂碳点（N,S-CDs）。N，S-CDs表现出与激发无关的光致发光行为，具有优异的稳定性。激发波长为420 nm时，565 nm处最大发射峰出现，量子产率（QY）为14.3%。发现维生素B12 （VB12）的吸收光谱与N，S-CDs的发射峰有很大程度的重叠，并基于VB12与N，S-CDs之间的内过滤效应（IFE），建立了药物中维生素B12的无标记灵敏检测方法。在最佳条件下，VB12浓度与反应体系在565 nm处的荧光猝灭度ΔF/F0在0.25 ~ 20 μM范围内呈良好的线性相关，检出限为77.5 nm。具有橙色荧光发射的N，S-CDs在生物分析领域具有巨大的应用潜力。
Nitrogen and sulfur co-doped carbon dots (N,S-CDs) with strong orange fluorescence were fabricated through a facile hydrothermal method using o-phenylenediamine and thiourea as precursors. The N,S-CDs exhibited excitation-independent photoluminescent behavior with excellent stability. The largest emission peak at 565 nm occurred at an excitation wavelength of 420 nm, and the quantum yield (QY) was 14.3%. It was found that the absorption spectra of vitamin B12 (VB12) overlaps with the emission peak of the N,S-CDs to a large extent, and based on the inner filter effect (IFE) between VB12 and N,S-CDs, a label-free and sensitive detection method of VB12 in drugs was established. Under optimal conditions, the concentration of VB12 exhibited a satisfactory linear correlation with the fluorescence quenching degree ΔF/F0 of the reaction system at 565 nm in the range of 0.25–20 μM, and the detection limit was 77.5 nM. The N,S-CDs with orange fluorescence emission show huge potential for use in the field of biological analysis.