Metagenomic analysis of the human distal gut microbiome
Metagenomic analysis of the human distal gut microbiome
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DOI：
10.1126/science.1124234
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发表时间：
2006-06-02
期刊：
影响因子：
56.9
通讯作者：
Nelson, Karen E.
中科院分区：
文献类型：
--
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
人类肠道微生物群由10（13）至10（14）种微生物组成，其集体基因组（“微生物组”）包含的基因至少是我们自己基因组的100倍。我们分析了相似的78万碱基对的独特的DNA序列和2062聚合酶链反应扩增的16 S核糖体DNA序列，从两个健康成人的粪便DNA。通过对已识别基因的代谢功能分析，我们将人类基因组与先前测序的微生物基因组的平均含量进行了比较。我们的微生物组具有显著富集的聚糖、氨基酸和异生物质的代谢;甲烷生成;以及2-甲基-D-β 4-磷酸途径介导的维生素和类异戊二烯的生物合成。因此，人类是一种超级有机体，其新陈代谢代表了微生物和人类属性的融合。
The human intestinal microbiota is composed of 10(13) to 10(14) microorganisms whose collective genome ("microbiome'') contains at least 100 times as many genes as our own genome. We analyzed similar to 78 million base pairs of unique DNA sequence and 2062 polymerase chain reaction amplified 16S ribosomal DNA sequences obtained from the fecal DNAs of two healthy adults. Using metabolic function analyses of identified genes, we compared our human genome with the average content of previously sequenced microbial genomes. Our microbiome has significantly enriched metabolism of glycans, amino acids, and xenobiotics; methanogenesis; and 2-methyl-D-erythritol 4-phosphate pathway - mediated biosynthesis of vitamins and isoprenoids. Thus, humans are superorganisms whose metabolism represents an amalgamation of microbial and human attributes.