The effect of melatonin on bacterial translocation following ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion.
The effect of melatonin on bacterial translocation following ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion.
复制标题
褪黑激素对肠系膜上动脉闭塞大鼠模型中缺血/再灌注损伤后细菌易位的影响。
DOI：
10.1186/s12893-015-0003-7
复制
发表时间：
2015-03-08
期刊：
影响因子：
1.9
通讯作者：
Berber I
中科院分区：
文献类型：
--
作者：
Ozban M;Aydin C;Cevahir N;Yenisey C;Birsen O;Gumrukcu G;Aydin B;Berber I
急性肠系膜缺血是一种危及生命的血管急症，由于缺血-再灌注损伤导致组织破坏。褪黑激素是松果体的主要激素，是活性氧（ROS）的强力清除剂，包括羟基和过氧自由基，以及单线态氧和一氧化氮。本研究旨在探讨褪黑激素是否能预防大鼠上级肠系膜缺血再灌注对肠组织的损害。将大鼠随机分为三组，每组10只。I组：分离上级肠系膜动脉（SMA），但不阻断;在组II和组III中，SMA在主动脉远端立即闭塞60分钟。之后，移除钳夹并开始再灌注期。在组III中，在再灌注开始前30分钟，腹膜内给予10 mg/kg褪黑激素。再灌注24小时后处死动物。收集组织样品以评价I/R诱导的肠损伤和细菌移位（BT）。组II中髓过氧化物酶活性、丙二醛水平和细菌易位发生率有统计学显著性增加，沿着谷胱甘肽水平降低。发现这些研究的参数在褪黑激素处理的动物（组III）中被标准化。我们的结论是，褪黑激素防止细菌移位，同时排除肠组织缺血/再灌注损伤的有害影响，在大鼠模型的上级肠系膜动脉闭塞。
Acute mesenteric ischemia is a life-threatening vascular emergency resulting in tissue destruction due to ischemia-reperfusion injury. Melatonin, the primary hormone of the pineal gland, is a powerful scavenger of reactive oxygen species (ROS), including the hydroxyl and peroxyl radicals, as well as singlet oxygen, and nitric oxide. In this study, we aimed to investigate whether melatonin prevents harmful effects of superior mesenteric ischemia-reperfusion on intestinal tissues in rats. Rats were randomly divided into three groups, each having 10 animals. In group I, the superior mesenteric artery (SMA) was isolated but not occluded. In group II and group III, the SMA was occluded immediately distal to the aorta for 60 minutes. After that, the clamp was removed and the reperfusion period began. In group III, 30 minutes before the start of reperfusion, 10 mg/kg melatonin was administered intraperitonally. All animals were sacrified 24 hours after reperfusion. Tissue samples were collected to evaluate the I/R-induced intestinal injury and bacterial translocation (BT). There was a statistically significant increase in myeloperoxidase activity, malondialdehyde levels and in the incidence of bacterial translocation in group II, along with a decrease in glutathione levels. These investigated parameters were found to be normalized in melatonin treated animals (group III). We conclude that melatonin prevents bacterial translocation while precluding the harmful effects of ischemia/reperfusion injury on intestinal tissues in a rat model of superior mesenteric artery occlusion.
登录
查看更多内容
影响因子：
3.3
作者：
Galano, Annia
通讯作者：
Galano, Annia
影响因子：
37.8
作者：
Nakamura, S;Sugiyama, S;Kugiyama, K
通讯作者：
Kugiyama, K
影响因子：
5.1
作者：
Ahsen, Ahmet;Gonul, Yucel;Yuksel, Seref
通讯作者：
Yuksel, Seref
影响因子：
2.9
作者：
Cuzzocrea, S;McDonald, MC;Thiemermann, C
通讯作者：
Thiemermann, C
影响因子：
--
作者：
Takhtfooladi, Hamed;Takhtfooladi, Mohammad;Mobarakeh, Sayed
通讯作者：
Mobarakeh, Sayed