Effect of glucagon-like peptide-1 and ghrelin on liver metabolites in steers.
Effect of glucagon-like peptide-1 and ghrelin on liver metabolites in steers.
复制标题
胰高血糖素样肽-1 和生长素释放肽对小公牛肝脏代谢物的影响。
DOI：
10.1071/an14363
复制
发表时间：
2014
影响因子：
1.4
通讯作者：
Taniguchi K.
中科院分区：
文献类型：
--
作者：
El-Sabagh M;Taniguchi D;Sugino T*;Obitsu T;Taniguchi K.
胰高血糖素样肽1（GLP-1）和ghrelin对非反刍动物的葡萄糖代谢具有相反的调节作用。然而，GLP-1和ghrelin调节营养分配的机制，特别是在肝脏中，在反刍动物中的表现要少得多。采用毛细管电泳飞行时间质谱（CE-TOFMS）结合多元统计分析的代谢组学方法研究GLP-1和Ghrelin对肉牛肝脏代谢的影响。采用3 × 3拉丁方设计，将3头荷斯坦牛（400 ± 5.0 kg LW）按日本饲养标准饲喂维持日粮，随机分为3组（GLP-1、ghrelin和生理盐水），每组间隔1周。在单次注射（1.0 μg/kg LW）GLP-1或生长素释放肽后30分钟进行肝活检，并通过Agilent CE-TOFMS系统分析代谢物。此外，还收集血液样本用于血浆激素分析。结果表明，GLP-1和ghrelin分别改变了20种和10种肝脏代谢产物（P < 0.05）。途径分析表明，GLP-1参与与糖酵解/脂肪生成、脂肪生成和肝脏脂质输出、氧化应激防御和蛋白质周转相关的生化途径。Ghrelin被证明参与糖酵解、蛋白质合成代谢和磷脂生物合成相关的途径。然而，胰岛素、生长激素和胰高血糖素的血浆浓度在治疗之间没有差异。这些结果意味着GLP-1和ghrelin参与了多生化途径，而不仅仅是简单地调节葡萄糖代谢。此外，GLP-1和ghrelin的作用可能分别独立于胰岛素和生长激素。
Glucagon-like peptide 1 (GLP-1) and ghrelin have opposite regulatory effects on glucose metabolism in non-ruminants. However, mechanisms by which GLP-1 and ghrelin regulate nutrient partitioning, particularly in the liver, have been much less demonstrated in ruminants. A novel metabolomic method based on capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) combined with multivariate statistical analysis was applied to address the GLP-1 and ghrelin-induced metabolic changes in the liver of steers. Three Holstein steers (400 ± 5.0 kg LW) fed a maintenance diet according to Japanese feeding standards were randomly assigned to three treatments (GLP-1, ghrelin and saline) in a 3 × 3 Latin square design with one week apart. Liver biopsies were taken 30 min after a single injection (1.0 μg/kg LW) of GLP-1 or ghrelin, and analysed for metabolites by Agilent CE-TOFMS system. Also, blood samples were collected for plasma hormones analysis. Results indicated that 20 and 10 liver metabolites were altered (P < 0.05) by GLP-1 and ghrelin, respectively. Pathway analysis showed that GLP-1 is involved in biochemical pathways related to glycolysis/gluconeogenesis, lipogenesis and lipid export from the liver, oxidative stress defence and protein turnover. Ghrelin was shown to be involved in pathways related to glycolysis, protein anabolism and phospholipid biosynthesis. However, plasma concentrations of insulin, growth hormone and glucagon did not differ between treatments. These results imply that GLP-1 and ghrelin are involved in multibiochemical pathways that go beyond simply regulating glucose metabolism. In addition, the effects of GLP-1 and ghrelin may potentially be independent of insulin and growth hormone, respectively.