A role for dystroglycan in basement membrane assembly
A role for dystroglycan in basement membrane assembly
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DOI：
10.1016/s0092-8674(00)81708-0
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发表时间：
1998-12-11
期刊：
影响因子：
64.5
通讯作者：
Campbell, KP
中科院分区：
文献类型：
--
作者：
Henry, MD;Campbell, KP
基底膜由典型的细胞外基质蛋白有序排列组成，但对这些结构在体内的组装知之甚少。我们研究了营养不良多糖的功能，这是一种细胞表面层粘连蛋白受体，由发育中和成年组织中接触基底膜的细胞表达。我们发现在类胚体中，营养不良多糖是形成基底膜所必需的。我们的结果进一步表明，营养不良蛋白-层粘连蛋白的相互作用是其他基底膜蛋白沉积的先决条件。抗肌营养不良多糖可能通过结合可溶性层粘连蛋白并将其组织在细胞表面而影响基底膜的组装。这些数据确定了营养不良葡聚糖在基底膜组装中的作用，并提出了这一过程的基本机制。
Basement membranes are composed of ordered arrays of characteristic extracellular matrix proteins, but little is known about the assembly of these structures in vivo. We have investigated the function of dystroglycan, a cell-surface laminin receptor expressed by cells contacting basement membranes in developing and adult tissues. We find that dystroglycan is required for the formation of a basement membrane in embryoid bodies. Our results further indicate that dystroglycan-laminin interactions are prerequisite for the deposition of other basement membrane proteins. Dystroglycan may exert its influence on basement membrane assembly by binding soluble laminin and organizing it on the cell surface. These data establish a role for dystroglycan in the assembly of basement membranes and suggest fundamental mechanisms underlying this process.