SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES
SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES
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DOI：
10.1126/science.1321498
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发表时间：
1992-07-10
期刊：
影响因子：
56.9
通讯作者：
SCHELLER, RH
中科院分区：
文献类型：
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作者：
BENNETT, MK;CALAKOS, N;SCHELLER, RH
突触囊泡储存在钙调节胞吐过程中释放的神经递质。神经递质释放的特异性要求突触囊泡和钙通道都定位于突触前活跃区。两个35千道尔顿蛋白（p35或syntaxins）被鉴定出与突触囊泡蛋白p65 （synaptotagmin）相互作用。p35蛋白仅在神经系统中表达，84%相同，包括羧基末端膜锚，并集中在突触部位的质膜上。一种针对p35免疫沉淀溶解型n型钙通道的抗体。p35蛋白可能在突触前活跃区钙通道附近的突触囊泡对接中起作用。
Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Two 35-kilodalton proteins (p35 or syntaxins) were identified that interact with the synaptic vesicle protein p65 (synaptotagmin). The p35 proteins are expressed only in the nervous system, are 84 percent identical, include carboxyl-terminal membrane anchors, and are concentrated on the plasma membrane at synaptic sites. An antibody to p35 immunoprecipitated solubilized N-type calcium channels. The p35 proteins may function in docking synaptic vesicles near calcium channels at presynaptic active zones.