Simultaneous spatial, conformational, and mass analysis of intact proteins and protein assemblies by nano-DESI travelling wave ion mobility mass spectrometry imaging
Simultaneous spatial, conformational, and mass analysis of intact proteins and protein assemblies by nano-DESI travelling wave ion mobility mass spectrometry imaging
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DOI：
10.1016/j.ijms.2021.116656
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发表时间：
2021-07-02
影响因子：
1.8
通讯作者：
Cooper, Helen J.
中科院分区：
文献类型：
--
作者：
Hale, Oliver J.;Hughes, James W.;Cooper, Helen J.
我们已经证明了天然纳米解吸电喷雾电离（纳米desi）质谱成像的蛋白质和蛋白质复合物在大鼠肾脏薄组织切片。在这里，我们展示了将行波离子迁移谱法（TWIMS）集成到原生纳米desi MSI工作流程中。TWIMS的好处是双重的：首先，到达时间滤波可以减少化学噪声，得到的离子图像显示更高的特异性。其次，结合TWIMS可以直接从成像数据集计算碰撞截面，从而测量蛋白质结构。我们的结果表明，需要使用加热入口的纳米desi测定的碰撞截面与使用环境温度入口的液体萃取表面分析（LESA）实验测定的碰撞截面与文献中可用的碰撞截面之间具有良好的一致性。离子图像和碰撞截面呈现了一系列蛋白质和蛋白质组件的分子量高达42.6 kDa。(c) 2021 Elsevier B.V.版权所有
We have previously demonstrated native nano-desorption electrospray ionization (nano-DESI) mass spectrometry imaging of proteins and protein complexes in thin tissue section of rat kidney. Here, we demonstrate the integration of travelling wave ion mobility spectrometry (TWIMS) into the native nanoDESI MSI workflow. The benefits of TWIMS are twofold: Firstly, arrival time filtering allows subtraction of chemical noise and the resulting ion images show improved specificity. Secondly, the incorporation of TWIMS enables the calculation of collision cross sections, and thus a measure of protein structure, directly from the imaging dataset. Our results show good agreement between the collision cross sections determined from nano-DESI, which requires the use of a heated inlet, and those determined experimentally from liquid extraction surface analysis (LESA) with an ambient temperature inlet, and those available in the literature. Ion images and collision cross sections are presented for a range of proteins and protein assemblies with molecular weights of up to 42.6 kDa. (c) 2021 Elsevier B.V. All rights reserved.