Genome-scale analysis of the metabolic networks of oleaginous Zygomycete fungi
Genome-scale analysis of the metabolic networks of oleaginous Zygomycete fungi
复制标题
产油接合菌的代谢网络的基因组规模分析。
DOI：
10.1016/j.gene.2013.03.012
复制
发表时间：
2013-05-25
期刊：
影响因子：
3.5
通讯作者：
Laoteng, Kobkul
中科院分区：
文献类型：
--
作者：
Vongsangnak, Wanwipa;Ruenwai, Rawisara;Laoteng, Kobkul
微生物脂正在成为食品和油脂化学品工业生产的一个有吸引力的选择。为了研究产油微生物的脂质生理，在系统水平上，利用生物信息学和系统生物学的方法构建了高山孢杆菌和环状毛霉的基因组代谢网络。作为专注于脂质产生的综合数据分析的支架，通过对发达代谢网络的比较分析，确定了控制脂肪添加合成、脂质储存和动员的一致代谢途径。在单个真菌中发现了独特的代谢特征，特别是在NADPH代谢和甾醇生物合成方面，这可能与真菌脂质表型的差异有关。本研究建立的框架详细描述了M. alpina和M. circinelloides之间的代谢关系，有助于进一步阐明微生物产油性，这可能导致作为油脂化工工业替代原料的微生物油的生产改进。(C) 2013 Elsevier B.V.版权所有
Microbial lipids are becoming an attractive option for the industrial production of foods and oleochemicals. To investigate the lipid physiology of the oleaginous microorganisms, at the system level, genome-scale metabolic networks of Mortierella alpina and Mucor circinelloides were constructed using bioinformatics and systems biology. As scaffolds for integrated data analysis focusing on lipid production, consensus metabolic routes governing fatty add synthesis, and lipid storage and mobilisation were identified by comparative analysis of developed metabolic networks. Unique metabolic features were identified in individual fungi, particularly in NADPH metabolism and sterol biosynthesis, which might be related to differences in fungal lipid phenotypes. The frameworks detailing the metabolic relationship between M. alpina and M. circinelloides generated in this study is useful for further elucidation of the microbial oleaginicity, which might lead to the production improvement of microbial oils as alternative feedstocks for oleochemical industry. (C) 2013 Elsevier B.V. All rights reserved.