Management of a Coxiella burnetii-infected sheep flock after an outbreak of Q fever in humans
Management of a Coxiella burnetii-infected sheep flock after an outbreak of Q fever in humans
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DOI：
10.3906/vet-1806-31
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发表时间：
2019-01-01
影响因子：
0.6
通讯作者：
Krt, Brane
中科院分区：
文献类型：
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作者：
Avbersek, Jana;Pate, Mateja;Krt, Brane
一群学生在一个牧羊场参加训练课程时感染Q热，其后当局对所涉及的羊群进行了详细调查。从478只羊中选择60只贝氏柯克斯体ELISA阳性和60只ELISA阴性的母羊进行试验，并分为4组。初始ELISA筛选后一个月，对羊群中的所有母羊（对照组除外）进行疫苗接种。依次收集的血液样本进行了测试，ELISA和PCR，粪便，牛奶，粪便，垫料和土壤进行了PCR检测。免疫应答率为92.7%，而总体C。暴发后鸡群中贝氏体的血清阳性率为64.9%。所有4个试验组动物的0.2%乳汁样本和34.4%粪便样本PCR呈阳性。C.在任何血液样品中均未检测到贝氏体DNA。粪便PCR阳性约35个月，从稳定的床上用品也是积极的，而牧场土壤样品是阴性的。看来，即使在短期内，广泛的清洁和消毒结合疫苗接种也可以被视为控制/预防农场环境中Q热的适当方法。
Following an outbreak of Q fever in a group of students who contracted the infection during a training course on a sheep farm, a detailed investigation of the sheep flock involved was conducted. Of 478 flock animals, 60 Coxiella burnetii ELISA-positive and 60 ELISA-negative ewes were selected for the trial and divided into four groups. A month after the initial ELISA screening, all ewes in the flock (except the control group) were vaccinated. Sequentially collected blood samples were tested with ELISA and PCR; feces, milk, manure, bedding, and soil were tested with PCR. The immune response to the vaccination was 92.7%, while the overall C. burnetii seroprevalence in the flock after the human outbreak was 64.9%. PCR was positive for 0.2% of milk samples and 34.4% of fecal samples of animals from all four trial groups. C. burnetii DNA was not detected in any of the blood samples. Manure was PCR-positive for about 35 months; bedding from the stable was also positive while samples of pasture soil were negative. It appears that extensive cleaning and disinfection combined with vaccination could be regarded as an appropriate approach to control/prevent Q fever in farm settings even in the short term.