The Inhibitory Effect of Thalidomide Analogue on Corneal Neovascularization in Rabbits
The Inhibitory Effect of Thalidomide Analogue on Corneal Neovascularization in Rabbits
复制标题
沙利度胺类似物对家兔角膜新生血管的抑制作用
作者：
You Kyung Lee;S. Chung
目的：观察沙利度胺类似物CC-3052对兔角膜新生血管的抑制作用。方法：在15只兔角膜基质中用丝线缝合诱导角膜新生血管。术后1周将30只眼分为5组，每组6只眼。三个组用3种不同浓度的局部CC-3052处理：0.25%（组1）、0.5%（组2）和1.0%（组3）。所有治疗每天进行两次，持续一周。在第4组中，结膜下注射一次0.5%浓度的CC-3052。在第5组中，作为实验对照组，每天两次添加局部平衡盐溶液，持续一周。用数码相机拍摄兔角膜，并在手术显微镜下进行检查。一半的角膜标本进行组织病理学分析，另一半用于测量肿瘤坏死因子和血管内皮生长因子（VEGF）的mRNA的浓度，通过逆转录聚合酶链反应。结果：与对照组相比，所有治疗组的新生血管面积均减少。对照组和所有治疗组之间的角膜新生血管百分比和评分存在显著差异。对照组的炎症、成纤维细胞、新生血管形成和抗VEGF抗体强度显著较低。对照组VEGF和肿瘤坏死因子浓度明显低于对照组。治疗组间无差异。结论：发现局部和结膜下施用沙利度胺类似物CC-3052可有效抑制角膜新生血管形成。
Purpose: To evaluate the effect of thalidomide analogue CC-3052 on corneal neovascularization in the rabbit model. Methods: Corneal neovascularization was induced in 15 rabbits by a silk suture in the corneal stroma. At 1 week after suturing, 30 eyes were divided into 5 groups of 6 eyes each. Three groups were treated with topical CC-3052 at 3 different concentrations: 0.25% (group 1), 0.5% (group 2), and 1.0% (group 3). All treatments were performed twice a day for a week. A 0.5% concentration of CC-3052 was injected subconjunctivally once in group 4. In group 5, a topical balanced salt solution was added twice a day for a week as the experimental control group. Rabbit corneas were photographed by a digital camera and examined by the operating microscope. Half of the corneal specimens were analyzed histopathologically, and the other half were used to measure the concentration of tumor necrosis factor &agr; and vascular endothelial growth factor (VEGF) messenger RNA by reverse transcriptase–polymerase chain reaction. Results: The neovascularized area was decreased in all treatment groups compared with the control group. There was a significant difference in the percentage and score of corneal neovascularization between the control and all treatment groups. Inflammation, fibroblast, neovascularization, and anti-VEGF antibody intensities were significantly lower in the control group. The concentration of VEGF and tumor necrosis factor &agr; was significantly lower in the control group. There was no difference between the treatment groups. Conclusions: Topical and subconjunctival administration of thalidomide analogue CC-3052 was found to be effective for the inhibition of corneal neovascularization.
影响因子：
5.1
作者：
Chang JH;Garg NK;Lunde E;Han KY;Jain S;Azar DT
通讯作者：
Azar DT
影响因子：
14.5
作者：
Philip, M;Rowley, DA;Schreiber, H
通讯作者：
Schreiber, H