A testis-specific serine protease, Prss41/Tessp-1, is necessary for the progression of meiosis during murine in vitro spermatogenesis
A testis-specific serine protease, Prss41/Tessp-1, is necessary for the progression of meiosis during murine in vitro spermatogenesis
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DOI：
10.1016/j.bbrc.2013.10.028
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发表时间：
2013-11-08
影响因子：
3.1
通讯作者：
Kimura, Atsushi P.
中科院分区：
文献类型：
--
作者：
Yoneda, Ryoma;Kimura, Atsushi P.
雄性减数分裂过程中蛋白酶的功能尚未得到充分研究。我们之前在小鼠睾丸中克隆并表征了四种睾丸特异性丝氨酸蛋白酶。其中一种蛋白酶 Prss41/Tessp-1 在生殖细胞和支持细胞中表达。这次，为了检查 Prss41/Tessp-1 在精子发生中的参与，我们在抗 Prss41/Tessp-1 抗体存在的情况下进行了睾丸碎片的器官培养。由于在支持细胞中，Prss41/Tessp-1 蛋白主要通过糖基磷脂酰肌醇与细胞内细胞器膜结合，因此该抗体有望影响精原细胞质膜上的 Prss41/Tessp-1。通过添加抗体，一些生精小管中的生殖细胞数量减少。标记基因的表达强烈表明精原细胞的减数分裂被阻止，并且通过末端脱氧核苷酸转移酶dUTP缺口末端标记测定，凋亡的生殖细胞的数量增加。这些数据表明Prss41/Tessp-1对于体外精子发生过程中精原细胞阶段减数分裂的进展是必需的。结合我们之前的研究，目前的结果表明 Prss/Tessp 蛋白酶对于减数分裂每个阶段的进展都很重要。 (C) 2013 Elsevier Inc. 保留所有权利。
The function of protease during male meiosis has not been well studied. We previously cloned and characterized four testis-specific serine proteases in the mouse testis. One of the proteases, Prss41/Tessp-1, was expressed in the germ and Sertoli cell. This time, to examine the involvement of Prss41/Tessp-1 in spermatogenesis, we conducted the organ culture of testis fragments in the presence of the anti-Prss41/Tessp-1 antibody. Because in the Sertoli cell, the Prss41/Tessp-1 protein was mostly associated with the membrane of intracellular organelles by glycosylphosphatidylinositol, the antibody was expected to affect Prss41/Tessp-1 at the plasma membrane of spermatogonia. By adding the antibody, the number of germ cells was decreased in some seminiferous tubules. The marker genes expression strongly suggested that meiosis was arrested at spermatogonia, and the number of apoptotic germ cells increased by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. These data indicated that Prss41/Tessp-1 was necessary for the progression of meiosis at the stage of spermatogonia during in vitro spermatogenesis. Together with our previous study, the current results suggest that the Prss/Tessp proteases are important for the progression of meiosis at each stage. (C) 2013 Elsevier Inc. All rights reserved.