REQUIREMENT FOR BDNF IN ACTIVITY-DEPENDENT SURVIVAL OF CORTICAL-NEURONS
REQUIREMENT FOR BDNF IN ACTIVITY-DEPENDENT SURVIVAL OF CORTICAL-NEURONS
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DOI：
10.1126/science.7907431
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发表时间：
1994-03-18
期刊：
影响因子：
56.9
通讯作者：
GREENBERG, ME
中科院分区：
文献类型：
--
作者：
GHOSH, A;CARNAHAN, J;GREENBERG, ME
利用体外培养的大鼠胚胎皮层神经元，探讨活动依赖性神经元存活的机制。激活电压敏感钙通道（VSCCs）可提高细胞存活率，而激活n -甲基- d -天冬氨酸受体则不能提高细胞存活率。这些作用与这两类钙通道诱导脑源性神经营养因子（BDNF）的表达有关。BDNF抗体（阻断BDNF的细胞内信号传导，但不阻断神经生长因子NT3或NT4/5）降低皮质神经元的存活，逆转vscc介导的存活增加。因此，内源性BDNF是皮层神经元的一种营养因子，其表达受vscc调节，并在这些神经元的vscc依赖性存活中发挥作用。
Cultured embryonic cortical neurons from rats were used to explore mechanisms of activity-dependent neuronal survival. Cell survival was increased by the activation of voltage-sensitive calcium channels (VSCCs) but not by activation of N-methyl-D-aspartate receptors. These effects correlated with the expression of brain-derived neurotrophic factor (BDNF) induced by these two classes of calcium channels. Antibodies to BDNF (which block intracellular signaling by BDNF, but not by nerve growth factor, NT3, or NT4/5) reduced the survival of cortical neurons and reversed the VSCC-mediated increase in survival. Thus, endogenous BDNF is a trophic factor for cortical neurons whose expression is VSCC-regulated and that functions in the VSCC-dependent survival of these neurons.