Fabrication of 3D biocompatible/biodegradable micro-scaffolds using dynamic mask projection microstereolithography
Fabrication of 3D biocompatible/biodegradable micro-scaffolds using dynamic mask projection microstereolithography
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DOI：
10.1016/j.jmatprotec.2009.05.004
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发表时间：
2009-08-01
影响因子：
6.3
通讯作者：
Chung, Ildoo
中科院分区：
文献类型：
--
作者：
Choi, Jae-Won;Wicker, Ryan;Chung, Ildoo
微立体光刻（mu SL）技术可以制造具有受控生化和机械微结构的三维（3D）组织工程支架。开发了用于组织工程的 mu SL 系统，使用数字微镜装置 (DMD (TM)) 进行动态图案生成，并使用 365 nm 过滤的紫外线 (UV) 灯交联光反应性聚合物溶液。 mu SL 系统的 x-y 分辨率类似于 2 μm，垂直 (z) 分辨率类似于 1 μm。为了证明 mu SL 在组织工程中的用途，合成了分子量接近 1200 Da 的聚富马酸丙酯 (PPF)。通过与富马酸二乙酯 (DEF) 以 7:3 (w/w) 的比例混合，PPF 的粘度降低至接近 150 cP（50 摄氏度）。最后，类似2%（w/w）的双（2,4,6-三甲基苯甲酰基）苯基氧化膦（BAPO）被添加到溶液中作为光引发剂。进行固化深度实验以确定合成的PPF的固化特性，并使用所得体系和预聚物构建具有类似于100μm的互连孔的3D多孔支架。微结构的扫描电子显微镜 (SEM) 和微计算机断层扫描 (mu CT) 图像表明，开发的 mu SL 系统是一种有前景的技术，可用于生产具有完全互连孔的可生物降解和生物相容性 3D 微支架。 (C) 2009 Elsevier B.V. 保留所有权利。
Microstereolithography (mu SL) technology can fabricate three-dimensional (3D) tissue engineered scaffolds with controlled biochemical and mechanical micro-architectures. A mu SL system for tissue engineering was developed using a Digital Micromirror Device (DMD (TM)) for dynamic pattern generation and an ultraviolet (UV) lamp filtered at 365 nm for crosslinking the photoreactive polymer solution. The mu SL system was designed with x-y resolution of similar to 2 mu m and a vertical (z) resolution of similar to 1 mu m. To demonstrate the use of mu SL in tissue engineering, poly(propylene fumarate) (PPF) was synthesized with a molecular weight of similar to 1200 Da. The viscosity of the PPF was reduced to similar to 150 cP (at 50 degrees C) by mixing with diethyl fumarate (DEF) in the ratio of 7:3 (w/w). Finally, similar to 2% (w/w) of bis(2,4,6-trimethylbenzoyl) phenylphosphine oxide (BAPO) was added to the solution to serve as a photoinitiator. Cure depth experiments were performed to determine the curing characteristics of the synthesized PPF, and the resulting system and prepolymer were used to construct a 3D porous scaffold with interconnected pores of similar to 100 mu m. Scanning electron microscopy (SEM), and micro-computed tomography (mu CT) images of the micro-architecture illustrate that the developed mu SL system is a promising technology for producing biodegradable and biocompatible 3D micro-scaffolds with fully interconnected pores. (C) 2009 Elsevier B.V. All rights reserved.