Gel observation chamber for rapid screening of root traits in cereal seedlings
Gel observation chamber for rapid screening of root traits in cereal seedlings
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DOI：
10.1023/b:plso.0000037029.82618.27
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发表时间：
2004-05-01
期刊：
影响因子：
4.9
通讯作者：
Forster, BP
中科院分区：
文献类型：
--
作者：
Bengough, AG;Gordon, DC;Forster, BP
描述了一种用于测量幼苗根部性状的简单凝胶室。幼苗位于塑料板上（至少其中一层是透明的）上的两层紧密间隔的透明凝胶平坦层之间。可以使用平板扫描仪以二维方式无损地记录根系统特征。容易测量的生根性状包括根长、伸长率、最长根、最深根、种子根数量和根角展开。野生大麦、地方品种大麦和栽培大麦的例子在凝胶室中、凝胶层之间或松散的土壤中生长。根系在凝胶平板上的生长与疏松土壤中的生长相似，栽培大麦的受精轴最多（约7根），根角展开最宽（约120度），野生大麦的受精轴最少（约3根），根角展开最窄（约40度）。测试的地方品种大麦品系介于野生大麦和现代栽培品种之间。进行单独的实验来研究谷粒质量和谷粒大小对这些生根性状的影响。这些实验包括遗传图谱群体的父母。在现代品种 Chime 中，种子根的数量很大程度上取决于籽粒质量。颗粒大小显着影响现代品种 Derkado、育种系 B83-12/21/5 和地方品种 Tadmor 的根数，这表明在任何筛选过程中都应控制颗粒大小。
A simple gel chamber is described for measurement of seedling root traits. Seedlings are located between two closely spaced flat layers of transparent gel, on plastic plates (at least one of which is transparent). Root system traits can be non-destructively recorded in two-dimensions using a flatbed scanner. Easily measured rooting traits include root length, elongation rate, longest root, deepest root, seminal root number, and angular spread of roots. Examples of wild, landrace, and cultivated barleys were grown in the gel chambers, between gel layers or in loosely packed soil. Root growth on the gel plates was similar to that in loose soil, with the cultivated barley having the most seminal axes (about 7), and widest angular spread of roots (about 120degrees), and wild barley the fewest seminal axes (about 3), and narrowest angular spread of roots (about 40degrees). Landrace barley lines tested were intermediate between wild barley and modem cultivars. Separate experiments were performed to study the effect of grain mass and grain size on these rooting traits. These experiments included parents of genetic mapping populations. Seminal root number was most strongly dependent on grain mass in the modem cultivar Chime. Grain size significantly influenced root number in the modern cultivar Derkado, the breeding line B83-12/21/5, and a selection from a landrace Tadmor, suggesting that grain size should be controlled in any screening exercise.