Gonadotrophin inhibitory hormone depresses gonadotrophin α and follicle-stimulating hormone β subunit expression in the pituitary of the domestic chicken
Gonadotrophin inhibitory hormone depresses gonadotrophin α and follicle-stimulating hormone β subunit expression in the pituitary of the domestic chicken
复制标题
DOI：
10.1111/j.1365-2826.2005.01260.x
复制
发表时间：
2004-12-01
影响因子：
3.2
通讯作者：
Sharp, PJ
中科院分区：
文献类型：
--
作者：
Ciccone, NA;Dunn, IC;Sharp, PJ
体外研究表明，从鹌鹑下丘脑中分离出的一种新型 12 氨基酸 RF 酰胺肽是一种促性腺激素抑制激素 (GnIH)。本研究的目的是在家鸡中研究这一假设。向被剥夺巢穴的孵化母鸡注射 GnIH 未能降低血浆黄体生成素 (LH) 的浓度。在成年公鸡切块垂体的短期（120 分钟）培养物中添加 GnIH 会抑制卵泡刺激素 (FSH) 和 LH 释放，并抑制常见 α 和 FSHbeta 促性腺激素亚基 mRNA，但对 LHbeta 亚基 mRNA 没有影响。孵化（产蛋外）的下丘脑 GnIH mRNA 高于产蛋母鸡，但在产蛋年末，产蛋外和产蛋肉种鸡的下丘脑 GnIH mRNA 量没有显着差异。结论是禽类 GnIH 可能在控制家鸡促性腺激素合成和相关组成型释放中发挥作用。
Studies performed in vitro suggest that a novel 12 amino acid RF amide peptide, isolated from the quail hypothalamus, is a gonadotrophin inhibitory hormone (GnIH). The aim of the present study was to investigate this hypothesis in the domestic chicken. Injections of GnIH into nest-deprived incubating hens failed to depress the concentration of plasma luteinizing hormone (LH). Addition of GnIH to short-term (120 min) cultures of diced pituitary glands from adult cockerels depressed follicle-stimulating hormone (FSH) and LH release and depressed common alpha and FSHbeta gonadotrophin subunit mRNAs, with no effect on LHbeta subunit mRNA. Hypothalamic GnIH mRNA was higher in incubating (out-of-lay) than in laying hens, but there was no significant difference in the amount of hypothalamic GnIH mRNA in out-of-lay and laying, broiler breeder hens at the end of a laying year. It is concluded that avian GnIH may play a role in controlling gonadotrophin synthesis and associated constitutive release in the domestic chicken.