The Gut Bacterial Communities Associated with Lab-Raised and Field-Collected Ants of Camponotus fragilis (Formicidae: Formicinae)
The Gut Bacterial Communities Associated with Lab-Raised and Field-Collected Ants of Camponotus fragilis (Formicidae: Formicinae)
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与实验室饲养和现场采集的脆弱弓背蚁（蚁科：蚁亚科）相关的肠道细菌群落
DOI：
10.1007/s00284-014-0586-8
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发表时间：
2014-09-01
影响因子：
2.6
通讯作者：
Wheeler, Diana E.
中科院分区：
文献类型：
--
作者：
He, Hong;Wei, Cong;Wheeler, Diana E.
Camponotus是第二大的蚂蚁属，并且已知具有Blochmannia属的主要内共生细菌。然而，人们对食物和环境变化对这些蚂蚁肠道细菌群落的影响知之甚少。我们调查了实验室饲养的和野外采集的脆弱弓背蚁的肠道细菌群落，这种蚂蚁发现于美国西南部和墨西哥北方。我们确定了肠道细菌组成和分布之间的作物，中肠和后肠的两种类型的殖民地的差异。不同的检测方法和蚂蚁的来源不同，细菌种类的数量也不同。实验室饲养的蚂蚁产生了12和11种使用经典的微生物培养方法和小亚基rRNA基因（16 S rRNA）聚合酶链反应-限制性片段长度多态性分析，分别。用同样的方法，野外收集的蚂蚁只产生了4种和1-3种。实验室饲养的蚂蚁的肠道细菌种类大多数在作物，中肠和后肠之间分布不均匀，每个部分都有自己的优势细菌种类。醋杆菌属是作物中最突出的细菌类群，约占作物克隆文库的55%.中肠克隆库中的优势种为布氏藻，占中肠克隆库的90%左右.铜绿假单胞菌在后肠中占优势，占后肠克隆文库的98%以上.铜绿假单胞菌是所有三个切片中唯一共同的菌种。实验室饲养的蚂蚁和野外收集的蚂蚁之间的比较，以及与其他物种的比较，表明肠道细菌群落随当地环境和饮食而变化。这里鉴定的细菌物种最有可能是对宿主影响不大的寄生虫或对菌落健康有害的温和病原体。
Camponotus is the second largest ant genus and known to harbor the primary endosymbiotic bacteria of the genus Blochmannia. However, little is known about the effect of diet and environment changes on the gut bacterial communities of these ants. We investigated the intestinal bacterial communities in the lab-raised and field-collected ants of Camponotus fragilis which is found in the southwestern United States and northern reaches of Mexico. We determined the difference of gut bacterial composition and distribution among the crop, midgut, and hindgut of the two types of colonies. Number of bacterial species varied with the methods of detection and the source of the ants. Lab-raised ants yielded 12 and 11 species using classical microbial culture methods and small-subunit rRNA genes (16S rRNAs) polymerase chain reaction-restriction fragment-length polymorphism analysis, respectively. Field-collected ants yielded just 4 and 1-3 species using the same methods. Most gut bacterial species from the lab-raised ants were unevenly distributed among the crop, midgut, and hindgut, and each section had its own dominant bacterial species. Acetobacter was the prominent bacteria group in crop, accounting for about 55 % of the crop clone library. Blochmannia was the dominant species in midgut, nearly reaching 90 % of the midgut clone library. Pseudomonas aeruginosa dominated the hindgut, accounting for over 98 % of the hindgut clone library. P. aeruginosa was the only species common to all three sections. A comparison between lab-raised and field-collected ants, and comparison with other species, shows that gut bacterial communities vary with local environment and diet. The bacterial species identified here were most likely commensals with little effect on their hosts or mild pathogens deleterious to colony health.