Does the human placenta express the canonical cell entry mediators for SARS-CoV-2?
Does the human placenta express the canonical cell entry mediators for SARS-CoV-2?
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DOI：
10.7554/elife.58716
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发表时间：
2020-07-14
期刊：
影响因子：
7.7
通讯作者：
Gomez-Lopez, Nardhy
中科院分区：
文献类型：
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作者：
Pique-Regi, Roger;Romero, Roberto;Gomez-Lopez, Nardhy
由严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 引起的 2019 年冠状病毒病 (COVID-19) 大流行已影响了超过 1000 万人，其中包括孕妇。迄今为止，尚无一致证据证明 SARS-CoV-2 可以垂直传播。新型冠状病毒通常利用血管紧张素转换酶 2 (ACE2) 受体和丝氨酸蛋白酶 TMPRSS2 进入细胞。在此，基于我们之前的单细胞研究 (Pique-Regi et al., 2019)、另一项研究以及新的单细胞/核 RNA 测序数据，我们研究了整个妊娠期间胎盘以及妊娠晚期绒毛膜羊膜中 ACE2 和 TMPRSS2 的表达。我们报告说，胎盘中 ACE2 和 TMPRSS2 的共转录可以忽略不计，因此这不是 SARS-CoV-2 垂直传播的可能途径。相比之下，引起先天性感染的寨卡病毒和巨细胞病毒的受体在胎盘细胞类型中高度表达。这些数据表明胎盘最低程度地表达 SARS-CoV-2 的典型细胞进入介质。
The pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected more than 10 million people, including pregnant women. To date, no consistent evidence for the vertical transmission of SARS-CoV-2 exists. The novel coronavirus canonically utilizes the angiotensin-converting enzyme 2 (ACE2) receptor and the serine protease TMPRSS2 for cell entry. Herein, building upon our previous single-cell study (Pique-Regi et al., 2019), another study, and new single-cell/nuclei RNA-sequencing data, we investigated the expression of ACE2 and TMPRSS2 throughout pregnancy in the placenta as well as in third-trimester chorioamniotic membranes. We report that co-transcription of ACE2 and TMPRSS2 is negligible in the placenta, thus not a likely path of vertical transmission for SARS-CoV-2. By contrast, receptors for Zika virus and cytomegalovirus, which cause congenital infections, are highly expressed by placental cell types. These data show that the placenta minimally expresses the canonical cell-entry mediators for SARS-CoV-2.